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Comparative study of Bacillus thuringiensis Cry1Ia and Cry1Aa delta-endotoxins : activation process and toxicity against Prays oleae
(
Elsevier Inc.
, 2010 , Article)
Cry1Ia and Cry1Aa proteins exhibited toxicities against Prays oleae with LC(50) of 189 and 116 ng/cm(2), respectively. The ability to process Cry1Ia11 protoxin by trypsin, chymotrypsin and P. oleae larvae proteases was ...
Heterologous expression and secretion of an antifungal Bacillus subtilis chitosanase (CSNV26) in Escherichia coli
(
Springer-Verlag Berlin Heidelberg
, 2013 , Article)
The aims of the study were the production improvement, the purification, the characterization and the activity investigation of chitosanase CSNV26 of Bacillus subtilis (V26). The gene csnV26 encoding for this protein was ...
Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity
(
Academic Press
, 2021 , Article)
Bacillus thuringiensis subsp. israelensis (Bti) spherical parasporal crystal contains several insecticidal proteins used as environmentally safe alternative to toxic chemical pesticides. The exploration of a Bti strain ...
Pyocin QDD1: A highly thermostable bacteriocin produced by Pseudomonas aeruginosa QDD1 for the biocontrol of foodborne pathogens Staphylococcus aureus and Bacillus cereus
(
Elsevier Ltd
, 2022 , Article)
This study was designed to explore alternative antibacterial bioresource products, bacteriocins produced by local strain of Pseudomonas aeruginosa QDD1. This bacteriocin (termed as Pyocin) has high inhibitory activity ...
Characterization of tunisian Bacillus thuringiensis strains with abundance of kurstaki subspecies harbouring insecticidal activities against the lepidopteran insect ephestia kuehniella
(
Springer US
, 2010 , Article)
The study of 257 crystal-producing Bacillus thuringiensis isolates from bioinsecticide free soil samples collected from different sites in Tunisia, was performed by PCR amplification, using six primer pairs specific for ...
Improvement of Photorhabdus temperata bioinsecticides production in low-cost media through adequate fermentation technology
(
Wiley Subscription Services, Inc.
, 2012 , Article)
To develop a cost effective process for bioinsecticides production by Photorhabdus temperata, dissolved oxygen (DO) requirements were investigated in both the complex and the optimized media using diluted seawater as a ...
Amino acids Y229 and F603 are involved in Bacillus thuringiensis Cry1Ac delta-endotoxin stability and toxicity
(
Blackwell Publishing
, 2012 , Article)
Bacillus thuringiensis Cry1Ac toxin shares structurally five conserved blocs with the other δ-endotoxins. To study the role of some amino acids belonging to these regions, two mutations, Y(229) P and F(603) S, were introduced ...
Cry1Ac toxicity enhancement towards lepidopteran pest Ephestia kuehniella through its protection against excessive proteolysis
(
Elsevier
, 2016 , Article)
Bacillus thuringiensis has been extensively used in agroecosystems for four decades due to its high specific toxicity. Strategies based on B. thuringiensis proteins combinations for the improvement of its activity present ...
Vegetative insecticidal protein of Bacillus thuringiensis BLB459 and its efficiency against Lepidoptera
(
Elsevier Ltd
, 2017 , Article)
Bacillus thuringiensis strain BLB459 supernatant showed a promising activity against Lepidopteran pests with extremely damages in the larvae midgut. Investigations of the genes that encode secreted toxin demonstrated that ...
Toxin stability improvement and toxicity increase against dipteran and lepidopteran larvae of Bacillus thuringiensis crystal protein Cry2Aa
(
Wiley
, 2016 , Article)
BACKGROUND
Bacillus thuringiensis δ-endotoxins are the most widely used biopesticides for controlling economically important crop pests and disease vectors. Improving their efficacy is of great benefit. Here, an improvement ...