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المؤلفAhmad, Salma
المؤلفNazar, Hanan
المؤلفAlatieh, Nouralhuda
المؤلفAl-Mansoob, Maryam
المؤلفFarooq, Zainab
المؤلفYusuf, Muna
المؤلفOuhtit, Allal
تاريخ الإتاحة2021-10-18T08:15:43Z
تاريخ النشر2021
اسم المنشورQatar University Annual Research an Exhibition 2021 (quarfe)
الاقتباسAhmad S., Nazar H., Alatieh N., Al-Mansoob M., Farooq Z., Yusuf M., Ouhtit A., "Validation of Novel Transcriptional Targets that Underpin CD44-promoted breast cancer cell invasion", Qatar University Annual Research Forum and Exhibition (QUARFE 2021), Doha, 20 October 2021, https://doi.org/10.29117/quarfe.2021.0153
معرّف المصادر الموحدhttps://doi.org/10.29117/quarfe.2021.0153
معرّف المصادر الموحدhttp://hdl.handle.net/10576/24438
الملخصIntroduction: Breast cancer (BC) is the most common cancer worldwide, and metastasis is its worst aspect and the first cause of death. Metastasis is a multistep process, where an invasion is a recurring event. The process of BC cell invasion involves three major factors, including cell adhesion molecules (CAM), proteinases and Growth factors.CD44, a family of CAM proteins and the hyaluronic acid (HA) cell surface receptor, acts as cell differentiation, cell migration/invasion and apoptosis regulator. Rationale: We have previously established a tetracycline (Tet)-OFF-regulated expression system, both in vitro and in vivo (Hill et al, 2006). As a complementary approach, the highly metastatic MDA-MB-231 BC cells expressing high levels of endogenous CD44s (the standard form of CD44), was cultured in the presence and absence of 50 µg/ml of HA. RNA samples were isolated from both cell experimental models, and microarray analysis (12K CHIP from Affymetrix) was applied. More than 200 CD44s transcriptional target genes were identified and were sub-divided into groups of genes based on their function: cell motility, cytoskeletal organization, ability to degrade ECM, and cell survival. Hypothesis: Among these 200 identified genes, we selected seven genes (ICAP-1, KYNU, AHR, SIRT1, SRSF8, PRAD1, and SOD2) and hypothesized that based on evidence from literature, these genes are potential novel targets of CD44-downstream signaling mediating BC cell invasion. Specific Aims: Pursuant to this goal, we proposed the following objectives: 1- Structural validation of ICAP-1, KYNU, AHR, SIRT1, SRSF8, PRAD1 and SOD2 as novel transcriptional targets of CD44/HA-downstream signaling at both RNA and Protein level using reverse transcription polymerase chain reaction (RT-PCR) and Western Blot respectively. 2-Functional validation of ICAP-1, KYNU, AHR, SIRT1, SRSF8, PRAD1and SOD2 as novel transcriptional targets that underpin CD44-promoted BC cell migration using wound healing assay after the transfection with siRNA.Innovation/Consclusion: This study validated seven transcriptional targets of CD44/HA-downstream signaling promoting BC cell invasion. Ongoing experiments aim to dissect the signaling pathways that link CD44 activation by HA to the transcription of these seven genes.
اللغةen
الناشرQatar University Press
الموضوعBreast cancer
Tumor invasion and metastasis
CD44
Hyaluronan
Microarray
العنوانValidation of Novel Transcriptional Targets that Underpin CD44-promoted breast cancer cell invasion
النوعPoster
dc.accessType Open Access


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