Show simple item record

AuthorAhmad, Salma
AuthorNazar, Hanan
AuthorAlatieh, Nouralhuda
AuthorAl-Mansoob, Maryam
AuthorFarooq, Zainab
AuthorYusuf, Muna
AuthorOuhtit, Allal
Available date2021-10-18T08:15:43Z
Publication Date2021
Publication NameQatar University Annual Research an Exhibition 2021 (quarfe)
CitationAhmad S., Nazar H., Alatieh N., Al-Mansoob M., Farooq Z., Yusuf M., Ouhtit A., "Validation of Novel Transcriptional Targets that Underpin CD44-promoted breast cancer cell invasion", Qatar University Annual Research Forum and Exhibition (QUARFE 2021), Doha, 20 October 2021, https://doi.org/10.29117/quarfe.2021.0153
URIhttps://doi.org/10.29117/quarfe.2021.0153
URIhttp://hdl.handle.net/10576/24438
AbstractIntroduction: Breast cancer (BC) is the most common cancer worldwide, and metastasis is its worst aspect and the first cause of death. Metastasis is a multistep process, where an invasion is a recurring event. The process of BC cell invasion involves three major factors, including cell adhesion molecules (CAM), proteinases and Growth factors.CD44, a family of CAM proteins and the hyaluronic acid (HA) cell surface receptor, acts as cell differentiation, cell migration/invasion and apoptosis regulator. Rationale: We have previously established a tetracycline (Tet)-OFF-regulated expression system, both in vitro and in vivo (Hill et al, 2006). As a complementary approach, the highly metastatic MDA-MB-231 BC cells expressing high levels of endogenous CD44s (the standard form of CD44), was cultured in the presence and absence of 50 µg/ml of HA. RNA samples were isolated from both cell experimental models, and microarray analysis (12K CHIP from Affymetrix) was applied. More than 200 CD44s transcriptional target genes were identified and were sub-divided into groups of genes based on their function: cell motility, cytoskeletal organization, ability to degrade ECM, and cell survival. Hypothesis: Among these 200 identified genes, we selected seven genes (ICAP-1, KYNU, AHR, SIRT1, SRSF8, PRAD1, and SOD2) and hypothesized that based on evidence from literature, these genes are potential novel targets of CD44-downstream signaling mediating BC cell invasion. Specific Aims: Pursuant to this goal, we proposed the following objectives: 1- Structural validation of ICAP-1, KYNU, AHR, SIRT1, SRSF8, PRAD1 and SOD2 as novel transcriptional targets of CD44/HA-downstream signaling at both RNA and Protein level using reverse transcription polymerase chain reaction (RT-PCR) and Western Blot respectively. 2-Functional validation of ICAP-1, KYNU, AHR, SIRT1, SRSF8, PRAD1and SOD2 as novel transcriptional targets that underpin CD44-promoted BC cell migration using wound healing assay after the transfection with siRNA.Innovation/Consclusion: This study validated seven transcriptional targets of CD44/HA-downstream signaling promoting BC cell invasion. Ongoing experiments aim to dissect the signaling pathways that link CD44 activation by HA to the transcription of these seven genes.
Languageen
PublisherQatar University Press
SubjectBreast cancer
Tumor invasion and metastasis
CD44
Hyaluronan
Microarray
TitleValidation of Novel Transcriptional Targets that Underpin CD44-promoted breast cancer cell invasion
TypePoster
dc.accessType Open Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record