Effect of Short Time of SARS-CoV-2 Infection in Caco-2 Cells.
Author | Zupin, Luisa |
Author | Fontana, Francesco |
Author | Clemente, Libera |
Author | Ruscio, Maurizio |
Author | Ricci, Giuseppe |
Author | Crovella, Sergio |
Available date | 2022-04-26T08:00:08Z |
Publication Date | 2022-03-29 |
Publication Name | Viruses |
Identifier | http://dx.doi.org/10.3390/v14040704 |
Citation | Zupin, L.; Fontana, F.; Clemente, L.; Ruscio, M.; Ricci, G.; Crovella, S. Effect of Short Time of SARS-CoV-2 Infection in Caco-2 Cells. Viruses 2022, 14, 704. https://doi.org/10.3390/v14040704 |
Abstract | Coronavirus disease 19 (COVID-19) clinical manifestations include the involvement of the gastrointestinal tract, affecting around 10% of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected children. In the present work, the consequence of a short time of viral absorption (5, 15, 30 and 60 min) was tested on the Caco-2 intestinal epithelial cell line. Our findings show that Caco-2 cells are highly permissive to SARS-CoV-2 infection, even after 5 min of viral inoculation at a multiplicity of infection of 0.1. No cytopathic effect was evident during the subsequent 7 days of monitoring; nevertheless, the immunofluorescence staining for the viral nucleocapsid confirmed the presence of intracellular SARS-CoV-2. Our findings highlight the very short time during which SARS-CoV-2 is able to infect these cells in vitro. |
Sponsor | This research was funded by RC 15/2017, 03/2020, 47/2020 from IRCCS Burlo Garofolo/Italian Ministry of Health and by the QUCG-CAS-22/23-499 Collaborative Grant from Qatar University. |
Language | en |
Publisher | MDPI |
Subject | Caco-2 cell line SARS-CoV-2 short time of infection viral absorption |
Type | Article |
Issue Number | 4 |
Volume Number | 14 |
ESSN | 1999-4915 |
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Biological & Environmental Sciences [881 items ]
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COVID-19 Research [808 items ]