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AuthorZupin, Luisa
AuthorFontana, Francesco
AuthorClemente, Libera
AuthorRuscio, Maurizio
AuthorRicci, Giuseppe
AuthorCrovella, Sergio
Available date2022-04-26T08:00:08Z
Publication Date2022-03-29
Publication NameViruses
Identifierhttp://dx.doi.org/10.3390/v14040704
CitationZupin, L.; Fontana, F.; Clemente, L.; Ruscio, M.; Ricci, G.; Crovella, S. Effect of Short Time of SARS-CoV-2 Infection in Caco-2 Cells. Viruses 2022, 14, 704. https://doi.org/10.3390/v14040704
URIhttp://hdl.handle.net/10576/30433
AbstractCoronavirus disease 19 (COVID-19) clinical manifestations include the involvement of the gastrointestinal tract, affecting around 10% of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected children. In the present work, the consequence of a short time of viral absorption (5, 15, 30 and 60 min) was tested on the Caco-2 intestinal epithelial cell line. Our findings show that Caco-2 cells are highly permissive to SARS-CoV-2 infection, even after 5 min of viral inoculation at a multiplicity of infection of 0.1. No cytopathic effect was evident during the subsequent 7 days of monitoring; nevertheless, the immunofluorescence staining for the viral nucleocapsid confirmed the presence of intracellular SARS-CoV-2. Our findings highlight the very short time during which SARS-CoV-2 is able to infect these cells in vitro.
SponsorThis research was funded by RC 15/2017, 03/2020, 47/2020 from IRCCS Burlo Garofolo/Italian Ministry of Health and by the QUCG-CAS-22/23-499 Collaborative Grant from Qatar University.
Languageen
PublisherMDPI
SubjectCaco-2 cell line
SARS-CoV-2
short time of infection
viral absorption
TitleEffect of Short Time of SARS-CoV-2 Infection in Caco-2 Cells.
TypeArticle
Issue Number4
Volume Number14
ESSN1999-4915
dc.accessType Open Access


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