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AuthorAhmetaj-Shala, Blerina
AuthorMarei, Isra
AuthorKawai, Ryota
AuthorRothery, Stephen
AuthorPericleous, Charis
AuthorMohamed, Nura A.
AuthorGashaw, Hime
AuthorBokea, Kalliopi
AuthorSamuel, Jake
AuthorVandenheste, Annabelle
AuthorShala, Fisnik
AuthorKirkby, Nicholas S.
AuthorMitchell, Jane A.
Available date2022-05-17T07:13:27Z
Publication Date2021-08-23
Publication NameFrontiers in Cell and Developmental Biology
Identifierhttp://dx.doi.org/10.3389/fcell.2021.681347
CitationAhmetaj-Shala B, Marei I, Kawai R, Rothery S, Pericleous C, Mohamed NA, Gashaw H, Bokea K, Samuel J, Vandenheste A, Shala F, Kirkby NS and Mitchell JA (2021) Activation and Contraction of Human “Vascular” Smooth Muscle Cells Grown From Circulating Blood Progenitors. Front. Cell Dev. Biol. 9:681347. doi: 10.3389/fcell.2021.681347
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85114408638&origin=inward
URIhttp://hdl.handle.net/10576/30951
AbstractBlood outgrowth smooth muscle cells (BO-SMCs) offer the means to study vascular cells without the requirement for surgery providing opportunities for drug discovery, tissue engineering, and personalized medicine. However, little is known about these cells which meant that their therapeutic potential remains unexplored. Our objective was to investigate for the first time the ability of BO-SMCs and vessel-derived smooth muscle cells to sense the thromboxane mimetic U46619 by measuring intracellular calcium elevation and contraction. U46619 (10–6 M) increased cytosolic calcium in BO-SMCs and vascular smooth muscle cells (VSMCs) but not in fibroblasts. Increased calcium signal peaked between 10 and 20 s after U46619 in both smooth muscle cell types. Importantly, U46619 (10–9 to 10–6 M) induced concentration-dependent contractions of both BO-SMCs and VSMCs but not in fibroblasts. In summary, we show that functional responses of BO-SMCs are in line with VSMCs providing critical evidence of their application in biomedical research.
LanguageN/A
PublisherFrontiers Media
Subjectblood vessel
Subjectcalcium
Subjectcontraction
Subjectpersonalized medicine
Subjectsmooth muscle progenitor cells
TitleActivation and Contraction of Human “Vascular” Smooth Muscle Cells Grown From Circulating Blood Progenitors
TypeArticle
Volume Number9
ESSN2296-634X


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