Show simple item record

AuthorFarooq, M.
AuthorTaha, N. A.
AuthorButorac, R. R.
AuthorEvans, D. A.
AuthorElzatahry, A. A.
AuthorWadaan, M. A. M.
AuthorCowley, A. H.
Available date2022-05-22T09:12:27Z
Publication Date2015
Publication NameAsian Pacific Journal of Cancer Prevention
ResourceScopus
Identifierhttp://dx.doi.org/10.7314/APJCP.2015.16.16.7003
URIhttp://hdl.handle.net/10576/31383
AbstractBackground: Nanoparticles of gold and silver are offering revolutionary changes in the field of cancer therapy. N-heterocyclic carbene (NHC) metal complexes possess diverse biological activities and are being investigated as potential chemotherapeutic agents. The purpose of this study was to examine the cytotoxicity and possible mechanisms of action of two types of newly synthesized nanofiber composites containing BIAN N-heterocyclic gold carbene complexes in two types of human cancer cells, namely breast cancer (MCF7) and liver cancer (HepG2) cells and also in normal human embryonic kidney cells (HEK 293). Materials and Methods: Cytotoxicity was assessed by MTT cell viability assay and oxidative stress by checking the total glutathione level. Results: Both compounds affected the cell survival of the tested cell lines at very low concentrations (IC50 values in the micro molar range) as compared to a well-known anti-cancer drug, 5 fluorouracil. A 60-80% depletion in total glutathione level was detected in treated cells. Conclusions: Reduction in total glutathione level is one of the biochemical pathways for the induction of oxidative stress which in turn could be a possible mechanism of action by which these compounds induce cytotoxicity in cancer cell lines. The in vitro toxicity towards cancer cells found here means that these molecules could be potential anticancer candidates.
Languageen
PublisherAsian Pacific Organization for Cancer Prevention
Subjectacenaphthene derivative
Subjectantineoplastic agent
Subjectcarbene
Subjectcoordination compound
Subjectfluorouracil
Subjectglutathione
Subjectgold
Subjectheterocyclic compound
Subjectmethane
Subjectnanofiber
Subjectanalogs and derivatives
Subjectcell survival
Subjectdrug effects
SubjectHEK293 cell line
SubjectHepG2 cell line
Subjecthuman
SubjectIC50
SubjectMCF 7 cell line
Subjectmetabolism
Subjectoxidative stress
SubjectAcenaphthenes
SubjectAntineoplastic Agents
SubjectCell Survival
SubjectCoordination Complexes
SubjectFluorouracil
SubjectGlutathione
SubjectGold
SubjectHEK293 Cells
SubjectHep G2 Cells
SubjectHeterocyclic Compounds
SubjectHumans
SubjectInhibitory Concentration 50
SubjectMCF-7 Cells
SubjectMethane
SubjectNanofibers
SubjectOxidative Stress
TitleBIAN N-heterocyclic gold carbene complexes induced cytotoxicity in human cancer cells via upregulating oxidative stress
TypeArticle
Pagination7003-7006
Issue Number16
Volume Number16


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record