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Application of statistical experimental design for optimisation of bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium
(
Sociedade Brasileira de Microbiologia - SBM
, 2014 , Article)
In order to overproduce bioinsecticides production by a sporeless Bacillus thuringiensis strain, an optimal composition of a cheap medium was defined using a response surface methodology. In a first step, a Plackett-Burman ...
Electropolymerized phenol derivatives as permselective polymers for biosensor applications
(
The Royal Society of Chemistry
, 2015 , Article)
Amperometric biosensors are often coated with a polymeric permselective film to avoid electroactive
interference by reducing agents present in the target medium. Phenylenediamine and phenol monomers are commonly used to ...
Isolation of the Bacillus thuringiensis plasmid carrying Bacthuricin F4 coding genes and evidence of its conjugative transfer
(
The Journal of Infection in Developing Countries
, 2014 , Article)
INTRODUCTION:
Conjugation is an excellent natural mode of DNA transfer in vivo between bacteria, particularly when these conjugative elements carry technological traits such as bacteriocin encoding genes. In the present ...
Protective effects of ethyl acetate fraction of Lawsonia inermis fruits extract against carbon tetrachloride-induced oxidative damage in rat liver
(
SAGE Publications
, 2013 , Article)
This study aimed to investigate the antioxidant properties of different fractions obtained from the fruits of Lawsonia inermis, a widely used medicinal plant, against carbon tetrachloride (CCl4)-induced oxidative stress ...
Overproduction of Delta-Endotoxins by Sporeless Bacillusthuringiensis Mutants Obtained by Nitrous Acid Mutagenesis
(
Springer-Verlag
, 2011 , Article)
Asporogenic and oligosporogenic Bacillus thuringiensis mutants having the ability to overproduce insecticidal crystal protein were generated by using nitrous acid (50 mg/ml), as chemical mutagenic agent. Insecticidal crystal ...
Evidence of the Involvement of E358, A498 and C571 of a New Cry1Ac δ-endotoxin of Bacillus thuringiensis in its High Insecticidal Activity Against Ephestia kuehniella
(
Humana Press Inc
, 2010 , Article)
A new cry1Ac-type gene was cloned from Bacillus thuringiensis strain BLB1, sequenced and expressed. The deduced amino acid sequence of the polypeptide has a predicted molecular mass of 132.186 kDa. The amino acid sequence ...
Antifungal activities of an endophytic Pseudomonas fluorescens strain Pf1TZ harbouring genes from pyoluteorin and phenazine clusters
(
Springer Netherlands
, 2010 , Article)
Pseudomonas fluorescens Pf1TZ was inhibitory in vitro to a number of phytopathogenic fungi and could protect vine plantlets against Botrytis cinerea. Total protection was reached after 3 weeks of bacterial inoculation. The ...
Study of the Bacillus thuringiensis Vip3Aa16 histopathological effects and determination of its putative binding proteins in the midgut of Spodoptera littoralis
(
Elsevier Inc.
, 2011 , Article)
The bacterium Bacillus thuringiensis produces, at the vegetative stage of its growth, Vip3A proteins with activity against a broad spectrum of lepidopteran insects. The Egyptian cotton leaf worm (Spodoptera littoralis) is ...
Integration of a Recombinant Chitinase into Bacillus thuringiensis Parasporal Insecticidal Crystal
(
Springer-Verlag
, 2011 , Article)
Chitinases have been successfully used in combination with Bacillus thuringiensis delta-endotoxins forming crystals in order to enhance their insecticidal activities. In this context, we opted for promoting the chitinase ...
Heterologous expression and secretion of an antifungal Bacillus subtilis chitosanase (CSNV26) in Escherichia coli
(
Springer-Verlag Berlin Heidelberg
, 2013 , Article)
The aims of the study were the production improvement, the purification, the characterization and the activity investigation of chitosanase CSNV26 of Bacillus subtilis (V26). The gene csnV26 encoding for this protein was ...