Show simple item record

AuthorKhedher, Saoussen Ben
AuthorJaoua, Samir
AuthorZouari, Nabil
Available date2015-12-16T07:54:30Z
Publication Date2014-09-17
Publication NameBiotechnology Research Internationalen_US
CitationSaoussen Ben Khedher, Samir Jaoua, and Nabil Zouari, “Overcome of Carbon Catabolite Repression of Bioinsecticides Production by Sporeless Bacillus thuringiensis through Adequate Fermentation Technology,” Biotechnology Research International, vol. 2014, Article ID 698587, 8 pages, 2014.
ISSN2090-3138
ISSN2090-3146 (online)
URIhttp://dx.doi.org/10.1155/2014/698587
URIhttp://hdl.handle.net/10576/3961
AbstractThe overcoming of catabolite repression, in bioinsecticides production by sporeless Bacillus thuringiensisstrain S22 was investigated into fully controlled 3 L fermenter, using glucose based medium. When applying adequate oxygen profile throughout the fermentation period (75% oxygen saturation), it was possible to partially overcome the catabolite repression, normally occurring at high initial glucose concentrations (30 and 40 g/L glucose). Moreover, toxin production yield by sporeless strain S22 was markedly improved by the adoption of the fed-batch intermittent cultures technology. With 22.5 g/L glucose used into culture medium, toxin production was improved by about 36% when applying fed-batch culture compared to one batch. Consequently, the proposed fedbatch strategy was efficient for the overcome of the carbon catabolite repression. So, it was possible to overproduce insecticidal crystal proteins into highly concentrated medium
SponsorTunisian Ministry of Higher Education and Scientific Research.
Languageen
PublisherHindawi Publishing Corporation
SubjectCarbon Catabolite
SubjectBioinsecticides
SubjectSporeless Bacillus thuringiensis
TitleOvercome of carbon catabolite repression of bioinsecticides production by sporeless Bacillus thuringiensis through adequate fermentation technology
TypeArticle
Volume Number2014


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record