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AuthorElleuch, Jihen
AuthorZribi Zghal, Raida
AuthorLacoix, Marie Noel
AuthorChandre, Fabrice
AuthorTounsi, Slim
AuthorJaoua, Samir
Available date2015-12-16T11:11:43Z
Publication Date2015-07
Publication NameMicrobiological Researchen_US
Identifierhttp://dx.doi.org/10.1016/j.micres.2015.04.007
CitationEvidence of two mechanisms involved in Bacillus thuringiensis israelensis decreased toxicity against mosquito larvae: Genome dynamic and toxins stability, Elleuch, Jihen; Zribi Zghal, Raida; Lacoix, Marie Noël; Chandre, Fabrice; Tounsi, Slim et al., (2015) Microbiological Research, vol. 176, p. 48-54
ISSN0944-5013
ISSN1618-0623 (online)
URIhttp://hdl.handle.net/10576/3964
AbstractBiopesticides based on Bacillus thuringiensis israelensis are the most used and most successful around the world. This bacterium is characterized by a dynamic genome able to win or lose genetic materials which leads to a decrease in its effectiveness. The detection of such phenomena is of great importance to monitor the stability of B. thuringiensis strains in industrial production processes of biopesticides. New local B. thuringiensis israelensis isolates were investigated. They present variable levels of delta-endotoxins production and insecticidal activities against Aedes aegypti larvae. Searching on the origin of this variability, molecular and biochemical analyses were performed. The obtained results describe two main reasons of the decrease of B. thuringiensis israelensis insecticidal activity. The first reason was the deletion of cry4Aa and cry10Aa genes from the 128-kb pBtoxis plasmid as evidenced in three strains (BLB124, BLB199 and BLB506) among five. The second was the early degradation of Cry toxins by proteases in larvae midgut mainly due to some amino acids substitutions evidenced in Cry4Ba and Cry11Aa δ-endotoxins detected in BLB356. Before biological treatment based on B. thuringiensis israelensis, the studies of microflore in each ecosystem have a great importance to succeed pest management programs.
SponsorTunisian Ministry of Higher Education and Scientific Research.
Languageen
PublisherElsevier GmbH
SubjectBacillus thuringiensis israelensis
SubjectAedes aegypti
Subjectcry genes content
SubjectProteolysis
TitleEvidence of two mechanisms involved in Bacillus thuringiensis israelensis decreased toxicity against mosquito larvae: Genomedynamic and toxins stability
TypeArticle
Pagination48-54
Volume Number176


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