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AuthorBen Khedher, Saoussen
AuthorJaoua, Samir
AuthorZouari, Nabil
Available date2015-12-21T07:59:22Z
Publication Date2014-01-15
Publication NameBrazilian Journal of Microbiologyen_US
CitationBen Khedher S, Jaoua S, Zouari N., "Application of statistical experimental design for optimisation of bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium", Brazilian Journal of Microbiology, 2014 Jan 15;44(3):927-33.
ISSN1517-8382
ISSN1678-4405 (online)
URIhttp://hdl.handle.net/10576/3968
AbstractIn order to overproduce bioinsecticides production by a sporeless Bacillus thuringiensis strain, an optimal composition of a cheap medium was defined using a response surface methodology. In a first step, a Plackett-Burman design used to evaluate the effects of eight medium components on delta-endotoxin production showed that starch, soya bean and sodium chloride exhibited significant effects on bioinsecticides production. In a second step, these parameters were selected for further optimisation by central composite design. The obtained results revealed that the optimum culture medium for delta-endotoxin production consists of 30 g L(-1) starch, 30 g L(-1) soya bean and 9 g L(-1) sodium chloride. When compared to the basal production medium, an improvement in delta-endotoxin production up to 50% was noted. Moreover, relative toxin yield of sporeless Bacillus thuringiensis S22 was improved markedly by using optimised cheap medium (148.5 mg delta-endotoxins per g starch) when compared to the yield obtained in the basal medium (94.46 mg delta-endotoxins per g starch). Therefore, the use of optimised culture cheap medium appeared to be a good alternative for a low cost production of sporeless Bacillus thuringiensis bioinsecticides at industrial scale which is of great importance in practical point of view.
SponsorTunisian Ministry of Higher Education and Scientific Research.
Languageen
PublisherSociedade Brasileira de Microbiologia - SBM
SubjectSporeless Bacillus thuringiensis
SubjectDelta-endotoxins
SubjectCheap medium
SubjectOptimisation
Subjectresponse surface methodology
TitleApplication of statistical experimental design for optimisation of bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium
TypeArticle
Pagination927-933
Issue Number3
Volume Number44


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