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AuthorElleuch, Jihen
AuthorTounsi, Slim
AuthorBen Hassen, Najeh Belguith
AuthorLacoix, Marie Noel
AuthorChandre, Fabrice
AuthorJaoua, Samir
AuthorZribi Zghal, Raida
Available date2015-12-23T08:52:25Z
Publication Date2015-01
Publication NameJournal of Invertebrate Pathology
Citation"Characterisation of novel Bacillus thuringiensis isolates against Aedes aegypti (Diptera: Culicidae) and Ceratitis capitata (Diptera: Tephridae)", Elleuch, Jihen; Tounsi, Slim; Belguith Ben Hassen, Najeh; Lacoix, Marie Noël; Chandre, Fabrice et al., (2015), Journal of Invertebrate Pathology, vol. 124, p. 90-97
ISSN1096-0805 (online)
AbstractBacillus thuringiensis is successfully used in pest management strategies as an eco-friendly bioinsecticide. Isolation and identification of new strains with a wide variety of target pests is an ever growing field. In this paper, new B. thuringiensis isolates were investigated to search for original strains active against diptera and able to produce novel toxins that could be used as an alternative for the commercial H14 strain. Biochemical and molecular characterization revealed a remarkable diversity among the studied strains. Using the PCR method, cry4C/Da1, cry30Ea, cry39A, cry40 and cry54 genes were detected in four isolates. Three strains, BLB355, BLB196 and BUPM109, showed feeble activities against Aedes aegypti larvae. Interestingly, spore-crystal mixtures of BLB361, BLB30 and BLB237 were found to be active against Ceratitis capitata with an LC50 value of about 65.375, 51.735 and 42.972 μg cm(-2), respectively. All the studied strains exhibited important mortality levels using culture supernatants against C. capitata larvae. This suggests that these strains produce a wide range of soluble factors active against C. capitata larvae.
SponsorTunisian Ministry of Higher Education and Scientific Research
PublisherElsevier Inc.
SubjectBacillus thuringiensis
SubjectCeratits capitata
SubjectInsecticidal activity
SubjectPhylogenetic relationships
TitleCharacterisation of novel Bacillus thuringiensis isolates against Aedes aegypti (Diptera: Culicidae) and Ceratitis capitata (Diptera:Tephridae)
Volume Number124

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