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AuthorDriss, Fatma
AuthorTounsi, Slim
AuthorJaoua, Samir
Available date2015-12-24T05:59:59Z
Publication Date2011-04
Publication NameCurrent Microbiology
CitationDriss, F., Tounsi, S., & Jaoua, S. (2011), "Relationship Between Plasmid Loss and Gene Expression in Bacillus Thuringiensis", Current Microbiology, 62(4):1287–1293.
ISSN0343-8651
URIhttp://dx.doi.org/10.1007/s00284-010-9857-1
URIhttp://hdl.handle.net/10576/3982
AbstractThe large genome of Bacillus thuringiensis was shortened by provoking plasmid loss, and the effect of such loss on the expression of both chromosomal and plasmid-harboured genes was studied. It was evidenced that the genomic material shortening of B. thuringiensis allows for the improvement of the expression of some of the constructive genes. Indeed, it was shown that curing was an efficient tool to obtain mutant strains with better expression of chromosomal genes. In fact, totally cured strains, HD1Cry-B and BNS3Cry, showed higher chitinolytic and/or proteolytic activities than the corresponding wild and/or partially cured ones. Although total curing is drastic for plasmid-harboured gene expression, the authors obtained partially cured strains, Cur255 and CurHD1, with a doubled level of delta-endotoxin production, and a 1.5-fold improvement of the bacteriocin-specific activity, respectively. In addition, the latter strains showed better relative proteolytic activities.
SponsorTunisian Ministere de l’Enseignement Superieur et de la Recherche Scientifique (MESRS)
Languageen
PublisherSpringer-Verlag
SubjectBacteriocin Activity
Relative Proteolytic Activity
AU Ml
Coumermycin
Plasmid Profile
High Molecular Weight Plasmid
TitleRelationship between plasmid loss and gene expression in Bacillus thuringiensis
TypeArticle
Pagination1287-1293
Issue Number4
Volume Number62
ESSN1432-0991
dc.accessType Abstract Only


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