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المؤلفBen Khedher, Saoussen
المؤلفKamoun, Amel
المؤلفJaoua, Samir
المؤلفZouari, Nabil
تاريخ الإتاحة2015-12-24T07:47:47Z
تاريخ النشر2011-10
اسم المنشورNew Biotechnology
المعرّفhttp://dx.doi.org/10.1016/j.nbt.2011.01.008
الاقتباس"Improvement of Bacillus thuringiensis bioinsecticide production by sporeless and sporulating strains using response surface methodology", Ben Khedher, Saoussen; Kamoun, Amel; Jaoua, Samir; Zouari, Nabil, (2011) New Biotechnology vol. 28 (6):p. 705-712
الرقم المعياري الدولي للكتاب1871-6784
معرّف المصادر الموحدhttp://hdl.handle.net/10576/3986
الملخصStatistical experimental designs, involving a Plackett-Burman design followed by a rotatable central composite design were used to optimize the culture medium constituents for Bacillus thuringiensis bioinsecticide production. This was carried out by using firstly an asporogenic strain and extrapolated to some sporeless and sporulating strains. Initial screening of production parameters was performed and the variables with statistically significant effects on delta-endotoxin production were identified: glucose, glycerol, yeast extract and MnSO(4). These variables were selected for further optimization by response surface methodology. The obtained results revealed that the optimum culture medium for delta-endotoxin production consists of 22.5 g/l of glucose, 4.8g/l of glycerol, 5.8 g/l of yeast extract and 0.008 g/l of MnSO(4). Under these conditions, delta-endotoxin production was 2,130 and 2,260 mg/l into 250 and 1,000 ml flask respectively, which represent more than 38% improvement in toxin production over the basal medium (1,636 mg/l). Such medium composition was shown to be suitable for overproducing delta-endotoxins by sporeless and sporulating strains.
راعي المشروعTunisian Ministry of Higher Education, Scientific Research and Technology
اللغةen
الناشرElsevier B.V.
الموضوعsporeless
Bacillus thuringiensis
العنوانImprovement of Bacillus thuringiensis bioinsecticide production by sporeless and sporulating strains using response surface methodology
النوعArticle
الصفحات705-712
رقم العدد6
رقم المجلد28
ESSN1876-4347
dc.accessType Abstract Only


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