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AuthorBen Khedher, Saoussen
AuthorKamoun, Amel
AuthorJaoua, Samir
AuthorZouari, Nabil
Available date2015-12-24T07:47:47Z
Publication Date2011-10
Publication NameNew Biotechnologyen_US
Identifierhttp://dx.doi.org/10.1016/j.nbt.2011.01.008
Citation"Improvement of Bacillus thuringiensis bioinsecticide production by sporeless and sporulating strains using response surface methodology", Ben Khedher, Saoussen; Kamoun, Amel; Jaoua, Samir; Zouari, Nabil, (2011) New Biotechnology vol. 28 (6):p. 705-712
ISSN1871-6784
ISSN1876-4347 (online)
URIhttp://hdl.handle.net/10576/3986
AbstractStatistical experimental designs, involving a Plackett-Burman design followed by a rotatable central composite design were used to optimize the culture medium constituents for Bacillus thuringiensis bioinsecticide production. This was carried out by using firstly an asporogenic strain and extrapolated to some sporeless and sporulating strains. Initial screening of production parameters was performed and the variables with statistically significant effects on delta-endotoxin production were identified: glucose, glycerol, yeast extract and MnSO(4). These variables were selected for further optimization by response surface methodology. The obtained results revealed that the optimum culture medium for delta-endotoxin production consists of 22.5 g/l of glucose, 4.8g/l of glycerol, 5.8 g/l of yeast extract and 0.008 g/l of MnSO(4). Under these conditions, delta-endotoxin production was 2,130 and 2,260 mg/l into 250 and 1,000 ml flask respectively, which represent more than 38% improvement in toxin production over the basal medium (1,636 mg/l). Such medium composition was shown to be suitable for overproducing delta-endotoxins by sporeless and sporulating strains.
SponsorTunisian Ministry of Higher Education, Scientific Research and Technology
Languageen
PublisherElsevier B.V.
Subjectsporeless
SubjectBacillus thuringiensis
TitleImprovement of Bacillus thuringiensis bioinsecticide production by sporeless and sporulating strains using response surface methodology
TypeArticle
Pagination705-712
Issue Number6
Volume Number28


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