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AuthorDammak, Mariam
AuthorJaoua, Samir
AuthorTounsi, Slim
Available date2015-12-27T06:57:42Z
Publication Date2011-12
Publication NameBiotechnology Lettersen_US
CitationDammak, M., Jaoua, S., & Tounsi, S., (2011), "Construction of a Bacillus thuringiensis genetically- engineered strain harbouring the secreted Cry1Ia delta-endotoxin in its crystal", Biotechnology Letters, 33(12): 2367–2372.
ISSN1573-6776 (online)
ISSN0141-5492
URIhttp://dx.doi.org/10.1007/s10529-011-0716-3
URIhttp://hdl.handle.net/10576/3988
AbstractUnlike other Bacillus thuringiensis Cry proteins, Cry1 Ia does not form a crystal since it is a secreted delta-endotoxin. We have engineered a Cry1 Iac chimeric protein by substituting the C-terminal part of Cry1 Ia by the corresponding Cry1 Ac part. When expressed in an acrystalliferous B. thuringiensis strain, Cry1Iac did not crystallize, but when expressed in the crystalliferous strain BNS3, the chimeric protein co-crystallized with the endogenous Cry1 A delta-endotoxins forming a typical bipyramidal crystal. The integration of Cry1 Ia in the composition of the crystal of BNS3 led to an increase of its delta-endotoxin production (13%) and to an improvement (60%) of its toxicity against Agrotis ipsilon.
SponsorTunisian Ministry of Higher Education and Scientific Research.
Languageen
PublisherSpringer Netherlands
SubjectAgrotis ipsilon
SubjectBacillus thuringiensis
SubjectCry1Ia
SubjectC-terminal domain
SubjectCo-crystallization
SubjectProduction
SubjectToxicity
TitleConstruction of a Bacillus thuringiensis genetically-engineered strain harbouring the secreted Cry1Ia delta-endotoxin in its crystal
TypeArticle
Pagination2367-2372
Issue Number12
Volume Number32


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