PREPARATION AND CHARACTERIZATION OF NANOSTRUCTURE MATERIAL FOR BIOLOGICAL APPLICATION

Abstract

Currently, nanomaterial has gained much attention in medical applications. Two different nanostructured materials were synthesized for biomedical purposes in this approach. Firstly, a composite of bimetallic nanoparticles containing Ag and Ni supported on metal oxide (TiO2) was successfully fabricated by co-precipitation manner via NaBH4 as a strong reducing agent employed as a photocatalyst and disinfector. A 1 g/L of NiO/Ag/TiO2 nanocomposite was assessed towards the decomposition of dyes (Methylene blue (MB)), Aspirin, and Paracetamol as pharmaceutical waste with a reduction rate of (93.19%, 77%, and 68.4%, respectively) under visible light within 60 minutes. Furthermore, the effect of annealing was studied which represents a faster degradation of MB dye and higher efficiency reaching 95.92% under visible light within 60 minutes. The basic mechanism of the photodegradation of MB via NiO/Ag/TiO2 nanocomposite was outlined. In addition, even after three cycles, the catalyst showed a significant degrading efficiency reached 90.4%. Next, the nanoparticle was evaluated against two different strains of bacteria (E. coli and Staphylococcus aureus.) in both visible light and dark conditions. The composite shows a dose-dependent bacteriostatic in both bacteria and has a bactericidal effect on E. coli that are exposed to 1 g/L of the material. Second, the preparation of virus-like silica mesoporous nanoparticles (V-MSN) as a drug carrier was done in this work. Due to their biomedical application, it is essential to find the in-vivo model toxicity of this medical carrier. However, the drug has been found to be non-hazard for doses up to 1000 mg/L tested on the zebrafish embryos model.