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AuthorVirto, Leire
AuthorOdeh, Verónica
AuthorGarcia-Quismondo, Enrique
AuthorHerrera, David
AuthorPalma, Jesús
AuthorTamimi, Faleh
AuthorSanz, Mariano
Available date2023-05-02T10:19:18Z
Publication Date2023-02-27
Publication NameClinical Oral Implants Research
Identifierhttp://dx.doi.org/10.1111/clr.14055
CitationVirto, L., Odeh, V., Garcia‐Quismondo, E., Herrera, D., Palma, J., Tamimi, F., & Sanz, M. (2023). Electrochemical decontamination of titanium dental implants. An in vitro biofilm model study. Clinical Oral Implants Research.
ISSN0905-7161
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85150661911&origin=inward
URIhttp://hdl.handle.net/10576/42212
AbstractObjectives: The objective of this study is to study the effect of electrochemical treatment on biofilms developed on titanium dental implants, using a six-species in vitro model simulating subgingival oral biofilms. Materials and Methods: Direct electrical current (DC) of 0.75 V, 1.5 V, and 3 V (anodic polarization, oxidation processes) and of −0.75 V, −1.5 V, and -3 V (cathodic polarization, reduction processes) was applied between the working and the reference electrodes for 5 min on titanium dental implants, which have been previously inoculated with a multispecies biofilm. This electrical application consisted of a three-electrode system where the implant was the working electrode, a platinum mesh was the counter electrode, and an Ag/AgCl electrode was the reference. The effect of the electrical application on the biofilm structure and bacterial composition was evaluated by scanning electron microscopy and quantitative polymerase chain reaction. A generalized linear model was applied to study the bactericidal effect of the proposed treatment. Results: The electrochemical construct at 3 V and −3 V settings significantly reduced total bacterial counts (p <.05) from 3.15 × 106 to 1.85 × 105 and 2.92 × 104 live bacteria/mL, respectively. Fusobacterium nucleatum was the most affected species in terms of reduction in concentration. The 0.75 V and −0.75 V treatments had no effect on the biofilm. Conclusion: Electrochemical treatments had a bactericidal effect on this multispecies subgingival in vitro biofilm model, being the reduction more effective than the oxidative treatment.
SponsorITI (International Team for Implantology) Grant No. 1320_2018.
Languageen
PublisherWiley-Blackwell
Subjectbiofilm
dental implant
direct current
electrochemical
oxidation and reduction
peri-implantitis
TitleElectrochemical decontamination of titanium dental implants. An in vitro biofilm model study
TypeArticle
ESSN1600-0501
dc.accessType Open Access


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