The system will be going down for regular maintenance. Please save your work and logout.
The use of principle component analysis and MALDI-TOF MS for the differentiation of mineral forming: Virgibacillus and Bacillus species isolated from sabkhas
AuthorAbdel Samad, Rim
Al Disi, Zulfa
Mohammad Ashfaq, Mohammad Yousaf
Wahib, Sara Mohiddin
MetadataShow full item record
Occurrence of mineral forming and other bacteria in mats is well demonstrated. However, their high diversity shown by ribotyping has not been explained, although it could explain the diversity of formed minerals. Common biomarkers as well as phylogenic relationships are useful tools for clustering the isolates and the prediction of their potential role in the natural niche. In this study, a combination of MALDI-TOF MS with PCA was shown to be a powerful tool to categorize 35 mineral forming bacterial isolates isolated from Dohat Faishakh sabkha, northwest of Qatar (23 from decaying mats and 12 from living ones). The 23 strains from decaying mats belong to the Virgibacillus genus as identified by ribotyping and are shown to be highly involved in the formation of protodolomite and a diversity of minerals. They were used as internal references for the categorization of sabkha bacteria. Combination of the isolation of bacteria on selective mineral forming media, their MALDI TOF MS protein profiling and PCA analysis established their relationship in a phylloproteomic dendrogram based on protein biomarkers including m/z 4905, 3265, 5240, 6430, 7765, and 9815. PCA analysis clustered the studied isolates into 3 major clusters, showing strong correspondence to the 3 phylloproteomic groups that were established by the dendrogram. Both clustering analysis means have evidently demonstrated a relationship between known Virgibacillus strains and other related bacteria based on profiling of their synthesized proteins. Thus, larger populations of bacteria in mats can be easily screened for their potential to exhibit certain activities, which is of ecological, environmental and biotechnological significance.
- Biological & Environmental Sciences [759 items ]
Showing items related by title, author, creator and subject.
Swinging with the Jet Set: Analysis of Electromagnetic Fields Inside Jet Engines - From Numerical and Experimental Analysis to Statistical Analysis Krishna, Aparna; Khattab, Tamer; Abdelaziz, Aya F.; Guizani, Mohsen ( Institute of Electrical and Electronics Engineers Inc. , 2016 , Article)Analyzing electromagnetic propagation inside jet engine turbines is a challenging and important research topic due to its potential applications in both civil and military fields. Electromagnetic modeling of such complex ...
Performance analysis of noninvasive electrophysiological methods for the assessment of diabetic sensorimotor polyneuropathy in clinical research: a systematic review and meta-analysis with trial sequential analysis Haque, Fahmida; Reaz, Mamun Bin Ibne; Ali, Sawal Hamid Md; Arsad, Norhana; Chowdhury, Muhammad Enamul Hoque ( Nature Research , 2020 , Article)Despite the availability of various clinical trials that used different diagnostic methods to identify diabetic sensorimotor polyneuropathy (DSPN), no reliable studies that prove the associations among diagnostic parameters ...
Ali, Kashif; Dong, Hai; Bouguettaya, Athman; Erradi, Abdelkarim; Hadjidj, Rachid ( Institute of Electrical and Electronics Engineers Inc. , 2017 , Conference Paper)We propose a 'Sentiment Analysis as a Service' (SAaaS) framework that abstracts sentiments from social information services, analyses and transforms into useful information. We propose a dynamic service composition mechanism ...