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المؤلفMohamed Qasim, Al-Fahdawi
المؤلفAldoghachi, Ahmed Faris
المؤلفAlhassan, Fatah H.
المؤلفAl-Doghachi, Faris A.J.
المؤلفAlshwyeh, Hussah Abdullah
المؤلفRasedee, Abdullah
المؤلفAlnasser, Sulaiman Mohammed
المؤلفAl-Qubaisi, Mothanna Sadiq
المؤلفIbrahim, Wisam Nabeel
تاريخ الإتاحة2023-06-19T07:51:36Z
تاريخ النشر2023-05-31
اسم المنشورHeliyon
المعرّفhttp://dx.doi.org/10.1016/j.heliyon.2023.e15560
الاقتباسAl-Fahdawi, M. Q., Aldoghachi, A. F., Alhassan, F. H., Al-Doghachi, F. A., Alshwyeh, H. A., Rasedee, A., ... & Ibrahim, W. N. (2023). Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles. Heliyon, 9(5).
معرّف المصادر الموحدhttps://www.sciencedirect.com/science/article/pii/S2405844023027676
معرّف المصادر الموحدhttp://hdl.handle.net/10576/44547
الملخصSilver-doped magnesia nanoparticles (Ag/MgO) were synthesized using the precipitation method and characterized by various techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), Brunner-Emmett-Teller (BET) surface area measurements, and dispersive X-ray spectroscopy (EDX). The morphology of Ag/MgO nanoparticles was determined by transmission and scanning electron microscopy, which revealed cuboidal shaped nanoparticles with sizes ranging from 31 to 68 nm and an average size of 43.5 ± 10.6 nm. The anticancer effects of Ag/MgO nanoparticles were evaluated on human colorectal (HT29) and lung adenocarcinoma (A549) cell lines, and their caspase-3, -8, and -9 activities, as well as Bcl-2, Bax, p53, cytochrome C protein expressions were estimated. Ag/MgO nanoparticles showed selective toxicity towards HT29 and A549 cells while remaining relatively innocuous towards the normal human colorectal, CCD-18Co, and lung, MRC-5 cells. The IC50 values of Ag/MgO nanoparticles on the HT29 and A549 cells were found to be 90.2 ± 2.6 and 85.0 ± 3.5 μg/mL, respectively. The Ag/MgO nanoparticles upregulated caspase-3 and -9 activities, downregulated Bcl-2, upregulated Bax and p53 protein expressions in the cancer cells. The morphology of the Ag/MgO nanoparticle treated HT29 and A549 cells was typical of apoptosis, with cell detachment, shrinkage, and membrane blebbing. The results suggest that Ag/MgO nanoparticles induce apoptosis in cancer cells and exhibit potential as a promising anticancer agent.
راعي المشروعThe publication of this article was funded by the Qatar National Library.
اللغةen
الناشرElsevier
الموضوعAg/MgO nanoparticles
HT29 cell
A549 cell
Antiproliferative
Apoptosis
العنوانPhysicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles
النوعArticle
رقم العدد5
رقم المجلد9
Open Access user License http://creativecommons.org/licenses/by/4.0/
ESSN2405-8440
dc.accessType Open Access


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