Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles
المؤلف | Mohamed Qasim, Al-Fahdawi |
المؤلف | Aldoghachi, Ahmed Faris |
المؤلف | Alhassan, Fatah H. |
المؤلف | Al-Doghachi, Faris A.J. |
المؤلف | Alshwyeh, Hussah Abdullah |
المؤلف | Rasedee, Abdullah |
المؤلف | Alnasser, Sulaiman Mohammed |
المؤلف | Al-Qubaisi, Mothanna Sadiq |
المؤلف | Ibrahim, Wisam Nabeel |
تاريخ الإتاحة | 2023-06-19T07:51:36Z |
تاريخ النشر | 2023-05-31 |
اسم المنشور | Heliyon |
المعرّف | http://dx.doi.org/10.1016/j.heliyon.2023.e15560 |
الاقتباس | Al-Fahdawi, M. Q., Aldoghachi, A. F., Alhassan, F. H., Al-Doghachi, F. A., Alshwyeh, H. A., Rasedee, A., ... & Ibrahim, W. N. (2023). Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles. Heliyon, 9(5). |
الملخص | Silver-doped magnesia nanoparticles (Ag/MgO) were synthesized using the precipitation method and characterized by various techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), Brunner-Emmett-Teller (BET) surface area measurements, and dispersive X-ray spectroscopy (EDX). The morphology of Ag/MgO nanoparticles was determined by transmission and scanning electron microscopy, which revealed cuboidal shaped nanoparticles with sizes ranging from 31 to 68 nm and an average size of 43.5 ± 10.6 nm. The anticancer effects of Ag/MgO nanoparticles were evaluated on human colorectal (HT29) and lung adenocarcinoma (A549) cell lines, and their caspase-3, -8, and -9 activities, as well as Bcl-2, Bax, p53, cytochrome C protein expressions were estimated. Ag/MgO nanoparticles showed selective toxicity towards HT29 and A549 cells while remaining relatively innocuous towards the normal human colorectal, CCD-18Co, and lung, MRC-5 cells. The IC50 values of Ag/MgO nanoparticles on the HT29 and A549 cells were found to be 90.2 ± 2.6 and 85.0 ± 3.5 μg/mL, respectively. The Ag/MgO nanoparticles upregulated caspase-3 and -9 activities, downregulated Bcl-2, upregulated Bax and p53 protein expressions in the cancer cells. The morphology of the Ag/MgO nanoparticle treated HT29 and A549 cells was typical of apoptosis, with cell detachment, shrinkage, and membrane blebbing. The results suggest that Ag/MgO nanoparticles induce apoptosis in cancer cells and exhibit potential as a promising anticancer agent. |
راعي المشروع | The publication of this article was funded by the Qatar National Library. |
اللغة | en |
الناشر | Elsevier |
الموضوع | Ag/MgO nanoparticles HT29 cell A549 cell Antiproliferative Apoptosis |
النوع | Article |
رقم العدد | 5 |
رقم المجلد | 9 |
Open Access user License | http://creativecommons.org/licenses/by/4.0/ |
ESSN | 2405-8440 |
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