Pre-fusion structure of a human coronavirus spike protein
المؤلف | Kirchdoerfer, Robert N. |
المؤلف | Cottrell, Christopher A. |
المؤلف | Wang, Nianshuang |
المؤلف | Pallesen, Jesper |
المؤلف | Yassine, Hadi M. |
المؤلف | Turner, Hannah L. |
المؤلف | Corbett, Kizzmekia S. |
المؤلف | Graham, Barney S. |
المؤلف | McLellan, Jason S. |
المؤلف | Ward, Andrew B. |
تاريخ الإتاحة | 2016-07-21T10:20:49Z |
تاريخ النشر | 2015-03-03 |
اسم المنشور | Nature |
المعرّف | http://dx.doi.org/10.1038/nature17200 |
الاقتباس | (yassine, Hadi., Kirchdoerfer, Robert N., / Pre-fusion structure of a human coronavirus spike protein , Nature Publishing Group, 531, 7592, Nature, 2015. |
الرقم المعياري الدولي للكتاب | 0028-0836 |
الملخص | HKU1 is a human betacoronavirus that causes mild yet prevalent respiratory disease1, and is related to the zoonotic SARS2 and MERS3 betacoronaviruses, which have high fatality rates and pandemic potential. Cell tropism and host range is determined in part by the coronavirus spike (S) protein4, which binds cellular receptors and mediates membrane fusion. As the largest known class I fusion protein, its size and extensive glycosylation have hindered structural studies of the full ectodomain, thus preventing a molecular understanding of its function and limiting development of effective interventions. Here we present the 4.0 Å resolution structure of the trimeric HKU1 S protein determined using singleparticle cryo-electron microscopy. In the pre-fusion conformation, the receptor-binding subunits, S1, rest above the fusion-mediating subunits, S2, preventing their conformational rearrangement. Surprisingly, the S1 C-terminal domains are interdigitated and form extensive quaternary interactions that occlude surfaces known in other coronaviruses to bind protein receptors. These features, along with the location of the two protease sites known to be important for coronavirus entry, provide a structural basis to support a model of membrane fusion mediated by progressive S protein destabilization through receptor binding and proteolytic cleavage. These studies should also serve as a foundation for the structure-based design of betacoronavirus vaccine immunogens. |
راعي المشروع | NIH |
اللغة | en |
الناشر | Nature Publishing Group |
الموضوع | Coronavirus spike protein structure |
النوع | Article |
الصفحات | 118-121 |
رقم العدد | 7592 |
رقم المجلد | 531 |
ESSN | 1476-4687 |
الملفات في هذه التسجيلة
الملفات | الحجم | الصيغة | العرض |
---|---|---|---|
لا توجد ملفات لها صلة بهذه التسجيلة. |
هذه التسجيلة تظهر في المجموعات التالية
-
أبحاث مركز البحوث الحيوية الطبية [738 items ]
-
أبحاث فيروس كورونا المستجد (كوفيد-19) [832 items ]