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المؤلفKirchdoerfer, Robert N.
المؤلفCottrell, Christopher A.
المؤلفWang, Nianshuang
المؤلفPallesen, Jesper
المؤلفYassine, Hadi M.
المؤلفTurner, Hannah L.
المؤلفCorbett, Kizzmekia S.
المؤلفGraham, Barney S.
المؤلفMcLellan, Jason S.
المؤلفWard, Andrew B.
تاريخ الإتاحة2016-07-21T10:20:49Z
تاريخ النشر2015-03-03
اسم المنشورNature
المعرّفhttp://dx.doi.org/10.1038/nature17200
الاقتباس(yassine, Hadi., Kirchdoerfer, Robert N., / Pre-fusion structure of a human coronavirus spike protein , Nature Publishing Group, 531, 7592, Nature, 2015.
الرقم المعياري الدولي للكتاب0028-0836
معرّف المصادر الموحدhttp://hdl.handle.net/10576/4686
الملخصHKU1 is a human betacoronavirus that causes mild yet prevalent respiratory disease1, and is related to the zoonotic SARS2 and MERS3 betacoronaviruses, which have high fatality rates and pandemic potential. Cell tropism and host range is determined in part by the coronavirus spike (S) protein4, which binds cellular receptors and mediates membrane fusion. As the largest known class I fusion protein, its size and extensive glycosylation have hindered structural studies of the full ectodomain, thus preventing a molecular understanding of its function and limiting development of effective interventions. Here we present the 4.0 Å resolution structure of the trimeric HKU1 S protein determined using singleparticle cryo-electron microscopy. In the pre-fusion conformation, the receptor-binding subunits, S1, rest above the fusion-mediating subunits, S2, preventing their conformational rearrangement. Surprisingly, the S1 C-terminal domains are interdigitated and form extensive quaternary interactions that occlude surfaces known in other coronaviruses to bind protein receptors. These features, along with the location of the two protease sites known to be important for coronavirus entry, provide a structural basis to support a model of membrane fusion mediated by progressive S protein destabilization through receptor binding and proteolytic cleavage. These studies should also serve as a foundation for the structure-based design of betacoronavirus vaccine immunogens.
راعي المشروعNIH
اللغةen
الناشرNature Publishing Group
الموضوعCoronavirus
spike protein
structure
العنوانPre-fusion structure of a human coronavirus spike protein
النوعArticle
الصفحات118-121
رقم العدد7592
رقم المجلد531
ESSN1476-4687
dc.accessType Abstract Only


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