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AuthorNajlah, Mohammad
AuthorKadam, Alisha
AuthorWan, Ka-Wai
AuthorAhmed, Waqar
AuthorTaylor, Kevin M.G.
AuthorElhissi, Abdelbary M.A
Available date2016-10-23T08:01:02Z
Publication Date2016-06-15
Publication NameInternational Journal of Pharmaceutics
Identifierhttp://dx.doi.org/10.1016/j.ijpharm.2016.04.027
CitationMohammad Najlah, Alisha Kadam, Ka-Wai Wan, Waqar Ahmed, Kevin M.G. Taylor, Abdelbary M.A Elhissi, Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines, International Journal of Pharmaceutics, Volume 506, Issues 1–2, 15 June 2016, Pages 102-109
ISSN03785173
URIhttp://www.sciencedirect.com/science/article/pii/S0378517316303118
URIhttp://hdl.handle.net/10576/4914
AbstractThe aim of this study is to investigate using nanoemulsion formulations as drug-delivery vehicles of paclitaxel (PX), a poor water-soluble anticancer drug. Two commercially available nanoemulsion fat formulations (Clinoleic 20% and Intralipid 20%) were loaded with PX and characterised based on their size, zeta potential, pH and loading efficiency. The effect of formulation on the cytotoxicity of PX was also evaluated using MTT assay.The droplet size of the Clinoleic emulsion increased from 254.1nm to 264.7nm when paclitaxel (6mg/ml) was loaded into the formulation, compared to the drug-free formulation. Similarly, the droplet size of Intralipid increased from 283.3 to 294.6nm on inclusion of 6mg/ml paclitaxel. The Polydispersity Indexes (PDIs) of all the nanoemulsion formulations (Clinoleic and Intralipid) were less than 0.2 irrespective of paclitaxel concentration indicating that all nanoemulsion formulations used were homogeneously sized. The pH range for the Clinoleic formulations (7.1–7.5) was slightly higher than that of the Intralipid formulations (6.5–6.9). The zeta potential of linoleic had a greater negative value than that of Intralipid.Loading efficiencies for paclitaxel were 70.4–80.2% and 44.2–57.4% for Clinoleic and Intralipid formulations, respectively. Clinoleic loaded with paclitaxel decreased the viability of U87-MG cell to 6.4±2.3%, compared to Intralipid loaded with paclitaxel (21.29±3.82%). Both nanoemulsions were less toxic to the normal glial cells (SVG-P12), decreasing the cell viability to 25–35%. This study suggests that nanoemulsions are useful and potentially applicable vehicles of paclitaxel for treatment of glioma.
Languageen
PublisherElsevier, Ltd
SubjectDroplet size
Nanoemulsion
Paclitaxel
Stability
Anticancer
MTT assay
TitleNovel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines
TypeArticle
Pagination102-109
Issue Number1
Volume Number506
Open Access user License http://creativecommons.org/licenses/by/4.0/


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