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    Evaluation of the Cytotoxic Behavior of Fungal Extracellular Synthesized Ag Nanoparticles Using Confocal Laser Scanning Microscope

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    Date
    2016-03-03
    Author
    Salaheldin, Taher A.
    Husseiny, Sherif M.
    Al-Enizi, Abdullah M.
    Elzatahry, Ahmed
    Cowley, Alan H.
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    Abstract
    Silver nanoparticles have been synthesized by subjecting a reaction medium to a Fusarium oxysporum biomass at 28 °C for 96 h. The biosynthesized Ag nanoparticles were characterized on the basis of their anticipated peak at 405 nm using UV-Vis-NIR spectroscopy. Structural confirmation was evident from the characteristic X-ray diffraction (XRD) pattern, high-resolution transmission electron Microscopy (HRTEM) and the particle size analyzer. The Ag nanoparticles were of dimension 40 ± 5 nm and spherical in shape. The study mainly focused on using the confocal laser scanning microscope (CLSM) to examine the cytotoxic activities of fungal synthesized Ag nanoparticles on a human breast carcinoma cell line MCF7 cell, which featured remarkable vacuolation, thus indicating a potent cytotoxic activity.
    DOI/handle
    http://dx.doi.org/10.3390/ijms17030329
    http://hdl.handle.net/10576/4922
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    • Materials Science & Technology [‎318‎ items ]

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