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المؤلفFirouzamandi, Masoumeh
المؤلفMoeini, Hassan
المؤلفHosseini, Seyed Davood
المؤلفBejo, Mohd Hair
المؤلفOmar, Abdul Rahman
المؤلفMehrbod, Parvaneh
المؤلفEl Zowalaty, Mohamed E.
المؤلفWebster, Thomas J.
المؤلفIderis, Aini
تاريخ الإتاحة2016-11-01T10:43:08Z
تاريخ النشر2016-01-14
اسم المنشورInternational Journal of Nanomedicine
المعرّفhttps://dx.doi.org/10.2147/IJN.S92225
الاقتباسFirouzamandi M, Moeini H, Hosseini SD, et al. Preparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease. International Journal of Nanomedicine. 2016;11:259-267.
الرقم المعياري الدولي للكتاب1176-9114
معرّف المصادر الموحدhttp://hdl.handle.net/10576/4948
معرّف المصادر الموحدhttp://dx.doi.org/10.2147/IJN.S92225
الملخصPlasmid DNA (pDNA)-based vaccines have emerged as effective subunit vaccines against viral and bacterial pathogens. In this study, a DNA vaccine, namely plasmid internal ribosome entry site-HN/F, was applied in ovo against Newcastle disease (ND). Vaccination was carried out using the DNA vaccine alone or as a mixture of the pDNA and dextran-spermine (D-SPM), a nanoparticle used for pDNA delivery. The results showed that in ovo vaccination with 40 µg pDNA/egg alone induced high levels of antibody titer (P<0.05) in specific pathogen-free (SPF) chickens at 3 and 4 weeks postvaccination compared to 2 weeks postvaccination. Hemagglutination inhibition (HI) titer was not significantly different between groups injected with 40 µg pDNA + 64 µg D-SPM and 40 µg pDNA at 4 weeks postvaccination (P>0.05). Higher antibody titer was observed in the group immunized with 40 µg pDNA/egg at 4 weeks postvaccination. The findings also showed that vaccination with 40 µg pDNA/egg alone was able to confer protection against Newcastle disease virus strain NDIBS002 in two out of seven SPF chickens. Although the chickens produced antibody titers 3 weeks after in ovo vaccination, it was not sufficient to provide complete protection to the chickens from lethal viral challenge. In addition, vaccination with pDNA/D-SPM complex did not induce high antibody titer when compared with naked pDNA. Therefore, it was concluded that DNA vaccination with plasmid internal ribosome entry site-HN/F can be suitable for in ovo application against ND, whereas D-SPM is not recommended for in ovo gene delivery.
راعي المشروعMinistry of Science, Technology and Innovations (MOSTI), Malaysia, for the research grant (ERGS/1-2012/5527122), and Institute of Bioscience, Higher Institution Centre of Excellence (IBS HICoE) grant from the Ministry of Higher Education, Government of Malaysia
اللغةen
الناشرDove Press
الموضوعNewcastle disease
DNA vaccine
in ovo vaccination
Newcastle disease virus
dextran-spermine nanoparticle
hemagglutinin and fusion
العنوانPreparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease
النوعArticle
الصفحات259-267
رقم المجلد11
ESSN1178-2013
dc.accessType Open Access


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