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AuthorTammam, Salma N.
AuthorKhalil, Mahmoud A.F.
AuthorGawad, Eman Abdul
AuthorAlthani, Asma
AuthorZaghloul, Hosam
AuthorAzzazy, Hassan M.E.
Available date2017-02-13T08:12:47Z
Publication Date2017-05-15
Publication NameCarbohydrate Polymersen_US
CitationSalma N. Tammam, Mahmoud A.F. Khalil, Eman Abdul Gawad, Asma Althani, Hosam Zaghloul, Hassan M.E. Azzazy, Chitosan gold nanoparticles for detection of amplified nucleic acids isolated from sputum, Carbohydrate Polymers, Volume 164, 15 May 2017, Pages 57-63
ISSN0144-8617
URIhttp://hdl.handle.net/10576/5242
URIhttp://dx.doi.org/10.1016/j.carbpol.2017.01.051
AbstractA platform for nucleic acid detection employing chitosan and chitosan coated gold nanoparticles (AuNPs) was developed. Mycobacterium tuberculosis (MTB) was used as a model target. MTB DNA was extracted from sputum using simple total nucleic acid extraction. Following amplification of MTB DNA, chitosan and AuNPs were added to samples. Free chitosan conjugated non-target DNA in negative samples, avoiding AuNP-DNAinteraction and hence negative samples remained red. In positive samples, amplified DNA was capable of saturating free chitosan leading to AuNP aggregation where positive samples turned blue. Via visual color detection 15/16 MTB positive samples and 3/3 negative samples were correctly identified. This test is a 1-tube, 1-step assay reducing the risk of contamination in molecular laboratories and is a proof of concept on how chitosan; a cheap polymer could increase the sensitivity of AuNPs towards specific detection of nucleic acids without using target specific oligotargeters or expensive extraction kits.
Languageen
PublisherElsevier
SubjectChitosan
SubjectGold nanoparticles
SubjectNucleic acid detection
SubjectSputum samples
SubjectMycobacterium tuberculosis
TitleChitosan gold nanoparticles for detection of amplified nucleic acids isolated from sputum
TypeArticle
Pagination57-63
Volume Number164
ESSN1879-1344


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