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AuthorAl-Saadi, Tahani M.
AuthorEltai, Nahla O.
AuthorHamdi, Wedad S.
AuthorBakhsh, Fadheela Dad
AuthorAzazzy, Hassan
AuthorAl-Ansari, Nasser
AuthorAl-Thani, Asmaa
Available date2017-06-08T06:37:16Z
Publication Date2017-05-21
Publication NameAfrican Journal of Microbiology Researchen_US
Identifierhttp://dx.doi.org/10.5897/AJMR2017.8518
CitationTahani M. Al-Saadi, Nahla O. Eltai, Wedad S. Hamdi, Fadheela Dad Bakhsh, Hassan Azazzy, Nasser Al-Ansari and Asmaa Al-Thani, "Colorimetric Gold Nanoparticles-Based Assay For Direct Detection Of Clostridium Difficile In Clinical Isolates From Qatar" African Journal of Microbiology Research Vol.11(19), pp. 756-763, May 2017
ISSN1996-0808
URIhttp://www.academicjournals.org/journal/AJMR/article-abstract/C2D27E864442
URIhttp://hdl.handle.net/10576/5554
AbstractClostridium difficile infection (CDI) is a significant health problem worldwide. Control and prevention strategies of C. difficile horizontal transmission require assays with fast detection with high specificity and sensitivity. Conventional diagnostic methods are time consuming and costly for clinical field settings. This study aims to develop gold nanoparticles (AuNPs)-based assay for direct qualitative detection of the nucleic acid of C. difficile and its toxins. A colloidal solution of AuNPs with a diameter of 13±1 nm was prepared and characterized. The qualitative colorimetric AuNPs assay was developed for restricted genomic C. difficile DNA detection, and results were confirmed by PCR. One hundred and five positive C. difficile isolates were collected from patients with diarrheal diseases and tested using AuNPs based-assay. Ninety-six samples (91.4%) were detected positive using AuNPs based assay, as indicated by the color change from red to blue within 1 min. All ninety-six positive samples were positive for toxin B. In conclusion, nano-gold assay prototype was developed for direct and inexpensive detection of C. difficile. The developed prototypes are simple, sensitive, rapid and can substitute PCR-based detection. The developed assay may show potential in the clinical diagnosis of C. difficile, especially in developing countries as it is less costly as compared to the commercially available assays.
SponsorNPRP award (NPRP 4-1215-3-317) from the Qatar National Research Fund
Languageen
PublisherAcademic Journals
SubjectGold nanoparticles
SubjectClostridium difficile
Subjectcolorimetric assay
Subjectpolymerase chain reaction (PCR)
TitleColorimetric gold nanoparticles-based assay for direct detection of Clostridium difficile in clinical isolates from Qatar
TypeArticle
Pagination756-763
Issue Number19
Volume Number11


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