Acute and chronic toxicity assessments of 17β-estradiol (E2) and 17α-ethinylestradiol (EE2) on the calanoid copepod Acartia clausi: Effects on survival, development, sex-ratio and reproduction

Abstract

Estrogens, such as the 17β-estradiol (E2) and the 17α-ethinylestradiol (EE2), have been regarded as a global threat to aquatic ecosystems due to their pseudo-persistence, their high estrogenic activity and their toxicity towards non-target species. Data regarding their ecotoxicological effects on marine calanoid copepods are very scarce. In this study, the calanoid copepod Acartia clausi was used as a model organism for estrogens exposure in marine pelagic ecosystems. Lethal effects of estrogens on A. clausi life-stages (Embryos, one day old nauplii: N1, three day old nauplii: N3, copepodites: C1-C3 and adults: C6) were investigated using 48 h acute tests. Copepods showed stage-specific responses against E2 and EE2 acute exposure. The most resistant life stage was N1 with LC50 values > 1500 μg L-1 and >5000 μg L-1, respectively for E2 and EE2. For N3, C1-C3, and C6, sensitivity to estrogens decreased with age and survival was affected at concentrations above those detected in the environment reflecting low estrogens acute toxicity for these life stages. In contrast, embryonic stage revealed high vulnerability to E2 and EE2 acute effects. Embryos showed non-monotonic dose-response and hatching success was significantly reduced at low realistic concentrations of E2 (0.005, 0.5, and 5 μg L-1) and EE2 (0.05 and 5 μg L-1).Survival, development and sex ratio of A. clausi to EE2 exposure at 1 and 100 μg L-1 were also determined during a life cycle experiment. Fitness of the females of the generation F0 was evaluated by measuring lifespan, prosome length and egg production. The main observed effects were the decrease of females’ prosome length, the feminization of the population and the reduction of the egg production for the generation F0 at 100 μg L-1 of EE2. This concentration is above those reported in the environment indicating the tolerance of A. clausi to EE2 at environmentally relevant concentrations.