Engineering of mutants of Anabaena PCC7120 (cyanobacterium) constructed by DNA-plasmid transfer and UV-radiation resistant to the herbicide Diuron capable of ammonia production
Author | Mahasneh, I.A.K. |
Author | Tiwari, D.N. |
Author | Al-Thani, R.F. |
Author | Qandeel, M. |
Available date | 2024-08-07T05:44:14Z |
Publication Date | 2004 |
Publication Name | Biotecnologia Aplicada |
Resource | Scopus |
ISSN | 0864-4551 |
Abstract | Ten mutants of Anabaena PCC7120 were successfully constructed to express two genetic traits, ammonia production and the resistance to the Diuron herbicide, using transposon induced mutagenesis and UV-radiation. The ranges for heterocyst frequency and ammonia production of mutants were 4.2%-6.8% and 2.3-6.6 μmol mg dry wt-1 h-1 respectively. The herbicide-resistance threshold for all mutants was 2.0 mg L-1, above which the mutants started to decline in health as observed microscopically. The dry weight of the mutants reached 300-400 mg L-1 with growth patterns similar to those of the wild-type after 20 days of growth in a batch culture in the presence of Diuron. In contrast, the addition of herbicide to the wild-type has led to a drastic reduction in O2- evolution followed by cellular vaculation, breakdown of filaments and cell death within 24 h (data not shown; microscopic inspection). |
Language | en |
Publisher | Elfos Scientiae , Cuba |
Subject | Ammonia production Anabaena DNA-plasmid transfer Herbicide-resistant |
Type | Article |
Pagination | 13-15 |
Issue Number | 1 |
Volume Number | 21 |
Files in this item
This item appears in the following Collection(s)
-
Biological & Environmental Sciences [881 items ]