• English
    • العربية
  • العربية
  • Login
  • QU
  • QU Library
  •  Home
  • Communities & Collections
  • Help
    • Item Submission
    • Publisher policies
    • User guides
    • FAQs
  • About QSpace
    • Vision & Mission
View Item 
  •   Qatar University Digital Hub
  • Qatar University Institutional Repository
  • Academic
  • Research Units
  • Biomedical Research Center
  • Biomedical Research Center Research
  • View Item
  • Qatar University Digital Hub
  • Qatar University Institutional Repository
  • Academic
  • Research Units
  • Biomedical Research Center
  • Biomedical Research Center Research
  • View Item
  •      
  •  
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    S1.P170 A terpenoids-enriched fraction of Inula viscosa: its chemical composition and anti-cancerous effects against human lung cancer cells

    Thumbnail
    View/Open
    ICNPR24 abstract inula.png (1.012Mb)
    Date
    2024-07-13
    Author
    Seglab, Fatiha
    Abu Asali, Mazen Mounir
    Al-Yafei, Thoraya
    Hassan, Hassan
    Shaito, Abdullah A.
    Metadata
    Show full item record
    Abstract
    Triple negative breast cancer (TNBC) is the most aggressive subtype of breast cancer and has limited therapeutic options. Effects of Ziziphus nummularia against TNBC have not been investigated yet. An ethanolic extract of Ziziphus nummularia (Burm.f.) Wight & Arn. (ZNE) was prepared and chromatographically fractionated. Phytochemical composition of ZNE and its chromatographically isolated fraction (F6) was identified both qualitatively by spectrophotometric assays and analytically by HPLC-PDA-MS/MS. Effects of ZNE and F6 on the viability of several cancerous cell lines were tested by MTT assay. The anti-cancerous potential of ZNE and F6 was tested in vitro in MDA-MB-231 cells, a TNBC cell line. ZNE and F6 radical scavenging capacity was tested using DPPH assay, and their effects on reactive oxygen species (ROS) generation in vitro in cells by DCFDA staining. Propidium iodide-based FACS analysis was used for cell cycle analysis. Scratch wound healing and trans-well migration chamber assays were used to assess MDA-MB-231 cell migration and invasion. Western blotting analysis was used to analyse changes in the levels of cell cycle, apoptosis and autophagy proteins. Findings showed that ZNE and F6 reduced the viability of several cancerous cell lines including MDA-MB-231 cells. F6 decreased MDAMB-231 viability more than crude ZNE or F6. ZNE and F6 are rich in phytochemicals and HPLC-PDA-MS/MS analysis identified several metabolites that were previously reported to have anti-cancerous effects. Both ZNE and F6 showed potent antioxidant capacity in the DPPH assay, but promoted reactive oxygen species (ROS) production in MDA-MB-231 cells; an effect which was blunted by the antioxidant N-acetyl cysteine (NAC). NAC also blunted ZNE- and F6-induced reduction in TNBC cell viability. We also demonstrated that ZNE and F6 induced an arrest of the cell cycle, and triggered apoptosis- and autophagy-mediated cell death. ZNE and F6 inhibited metastasis-related cellular processes by modifying cell migration, invasion, and adhesion. Collectively, our findings reveal that Z. nummularia is rich in metabolites that can attenuate the malignant phenotype of TNBC and may provide novel approaches for the discovery of new drug leads for treatment of TNBC and other cancers.
    DOI/handle
    http://hdl.handle.net/10576/59132
    Collections
    • Biomedical Research Center Research [‎800‎ items ]

    entitlement


    Qatar University Digital Hub is a digital collection operated and maintained by the Qatar University Library and supported by the ITS department

    Contact Us | Send Feedback
    Contact Us | Send Feedback | QU

     

     

    Home

    Submit your QU affiliated work

    Browse

    All of Digital Hub
      Communities & Collections Publication Date Author Title Subject Type Language Publisher
    This Collection
      Publication Date Author Title Subject Type Language Publisher

    My Account

    Login

    Statistics

    View Usage Statistics

    About QSpace

    Vision & Mission

    Help

    Item Submission Publisher policiesUser guides FAQs

    Qatar University Digital Hub is a digital collection operated and maintained by the Qatar University Library and supported by the ITS department

    Contact Us | Send Feedback
    Contact Us | Send Feedback | QU

     

     

    Video