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المؤلفAlasaad, Samer
المؤلفSoriguer, Ramón C
المؤلفAbu-Madi, Marawan
المؤلفEl Behairy, Ahmed
المؤلفJowers, Michael J
المؤلفBaños, Pablo Díez
المؤلفPíriz, Ana
المؤلفFickel, Joerns
المؤلفZhu, Xing-Quan
تاريخ الإتاحة2018-01-22T08:01:16Z
تاريخ النشر2011-06-01
اسم المنشورVeterinary Parasitology
المعرّفhttp://dx.doi.org/10.1016/j.vetpar.2011.01.059
الاقتباسA TaqMan real-time PCR-based assay for the identification of Fasciola spp. S Alasaad, RC Soriguer, M Abu-Madi, A El Behairy, MJ Jowers, PD Baños et al. Veterinary parasitology , 179 (1), 266-271
معرّف المصادر الموحدhttp://hdl.handle.net/10576/6167
الملخصReal time quantitative PCR (qPCR) is one of the key technologies of the post-genome era, with clear advantages compared to normal end-point PCR. In this paper, we report the first qPCR-based assay for the identification of Fasciola spp. Based on sequences of the second internal transcribed spacers (ITS-2) of the ribosomal rRNA gene, we used a set of genus-specific primers for Fasciola ITS-2 amplification, and we designed species-specific internal TaqMan probes to identify F. hepatica and F. gigantica, as well as the hybrid 'intermediate'Fasciola. These primers and probes were used for the highly specific, sensitive, and simple identification of Fasciola species collected from different animal host from China, Spain, Niger and Egypt. The novel qPCR-based technique for the identification of Fasciola spp. may provide a useful tool for the epidemiological investigation of Fasciola infection, including their intermediate snail hosts.
اللغةen
الناشرElsevier
الموضوعReal time qPCRTaqMan probe
Parasite identification
Fasciola hepatica
The ‘intermediate’ Fasciola
العنوانA TaqMan real-time PCR-based assay for the identification of Fasciola spp.
النوعArticle
الصفحات266-271
رقم العدد1-3
رقم المجلد179
dc.accessType Abstract Only


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