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AuthorBoughattas, S
AuthorSharma, A
AuthorAl-Sadeq, D
AuthorKhader, S
AuthorFouladi, M
AuthorAbu-Alainin, W
AuthorAl-Ansari, K
AuthorIsmail, A
AuthorAl-Thani, A
AuthorAbu-Madi, M
Available date2018-01-22T09:31:53Z
Publication Date2017
Publication NameFrontiers in Cellular and Infection Microbiology
Identifierhttp://dx.doi.org/10.3389/fcimb.2017.00343
CitationBoughattas S, Behnke JM, Al-Ansari K, Sharma A, Abu-Alainin W, Al-Thani A and Abu-Madi MA (2017) Molecular Analysis of the Enteric Protozoa Associated with Acute Diarrhea in Hospitalized Children. Front. Cell. Infect. Microbiol. 7:343. doi: 10.3389/fcimb.2017.00343
IdentifierArticle number 434
URIhttp://hdl.handle.net/10576/6169
AbstractWorld Health Organization (WHO) has identified Cryptosporidium spp. as globally the most common diarrhea-causing protozoan. Its transmission occurs mainly through ingestion of water or food contaminated with the feces of an infected human or animal. Infected food handlers are also a major Cryptosporidium contamination risk. A wide diversity of Cryptosporidium spp. and subtypes infect humans and due to the host specificity of each species, the use of molecular techniques and genotyping of subject’s stool samples will help in identifying contamination source. Molecular prevalence was investigated using real-time PCR targeting a 214 base pairs fragment of 18s-rRNA. The analyzed populations consist of hospitalized diarrheic pediatrics and asymptomatic food handlers. The positive samples by RT-PCR were subjected to species identification by PCR-RFLP and GP60 gene sequencing. The parasite was detected by RT-PCR in 15% of the children and 4% of the food-handlers. Genotyping of Cryptosporidium spp. revealed a predominance of Cryptosporidium parvum. Sequence analysis of the GP60 locus in the present study has identified only C. parvum subtype family IId with mostly the IIdA20G1 subtype among the two populations. This study provides supplementary information for implementing prevention and control strategies to reduce the burden of these pediatric protozoan infections.
SponsorThis study was funded by the Qatar National Research Fund (QRNF) at Qatar Foundation through the National Priorities Research Program (Project No. NPRP8-1556-3-313).
Languageen
PublisherFrontiers Media
SubjectCryptosporidium
RT-PCR
genotyping
sequencing
pediatrics
foodhandlers
TitleMolecular identification and characterization of Cryptosporidium spp. among pediatric and food-handlers subjects in Qatar
TypeArticle
Volume Number7
ESSN2235-2988
dc.accessType Open Access


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