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AuthorMarei, Hany El Sayed
AuthorEl-Gamal, Aya
AuthorAlthani, Asma
AuthorAfifi, Nahla
AuthorAbd-Elmaksoud, Ahmed
AuthorFarag, Amany
AuthorCenciarelli, Carlo
AuthorThomas, Caceci
AuthorAnwarul, Hasan
Available date2018-02-08T06:59:56Z
Publication Date2018-02-01
Publication NameJournal of Cellular Physiology
Identifierhttp://dx.doi.org/10.1002/jcp.25937
CitationMarei HES, El-Gamal A, Althani A, et al. Cholinergic and dopaminergic neuronal differentiation of human adipose tissue derived mesenchymal stem cells. J Cell Physiol. 2018;233:936–945. https://doi.org/10.1002/jcp.25937
ISSN0021-9541
URIhttp://hdl.handle.net/10576/6260
AbstractMesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various cell types such as cartilage, bone, and fat cells. Recent studies have shown that induction of MSCs in vitro by growth factors including epidermal growth factor (EGF) and fibroblast growth factor (FGF2) causes them to differentiate into neural like cells. These cultures also express ChAT, a cholinergic marker; and TH, a dopaminergic marker for neural cells. To establish a protocol with maximum differentiation potential, we examined MSCs under three experimental culture conditions using neural induction media containing FGF2, EGF, BMP-9, retinoic acid, and heparin. Adipose-derived MSCs were extracted and expanded in vitro for 3 passages after reaching >80% confluency, for a total duration of 9 days. Cells were then characterized by flow cytometry for CD markers as CD44 positive and CD45 negative. MSCs were then treated with neural induction media and were characterized by morphological changes and Q-PCR. Differentiated MSCs expressed markers for immature and mature neurons; β Tubulin III (TUBB3) and MAP2, respectively, showing the neural potential of these cells to differentiate into functional neurons. Improved protocols for MSCs induction will facilitate and ensure the reproducibility and standard production of MSCs for therapeutic applications in neurodegenerative diseases.
Languageen
PublisherWiley
Subjectadipose tissue
cholinergic neurons
dopaminergic neurons
mesenchymal stem cell
TitleCholinergic and dopaminergic neuronal differentiation of human adipose tissue derived mesenchymal stem cells.
TypeArticle
Pagination12088-12088
Issue Number7
Volume Number234
ESSN1097-4652
dc.accessType Abstract Only


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