Effect of psoriasis linked CARMA2sh gene in transgenic animal model

Abstract

Background

• CARMA2 belongs to the CARMA family of proteins. They are involved in the regulation and activation of NF-κB, that has a central role in controlling the immune and inflammatory response, cell survival and proliferation.

• CARMA2short (CARMA2sh) is the most prominent CARMA2 isoform expressed in human keratinocytes

• It has already been identified that CARMA2sh induces activation of NF-κB in association with another CARD-containing protein, namely BCL10, and the adapter protein TRAF2.

• This study identified a CARMA Inhibitory Kinase(CIK) which inhibits the ability to induce NF-κB

• CIK is not tested for their function in Human Primary keratinocytes and hence we attempt to understand the function of CIK and its associated molecules by invitro & invivo models

• The inhibitory activity of CIK on CARMA2 in primary human keratinocytes expressing wild (wt) & mutant CARMA2 was analyzed