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AuthorAbid, Rohma
AuthorIslam, Muhammad
AuthorSaeed, Hamid
AuthorAhmad, Abrar
AuthorImtiaz, Fariha
AuthorYasmeen, Anam
AuthorRathore, Hassaan Anwer
Available date2022-10-25T12:18:58Z
Publication Date2022-09-30
Publication NameFrontiers in Pharmacology
Identifierhttp://dx.doi.org/10.3389/fphar.2022.996755
CitationAbid R, Islam M, Saeed H, Ahmad A, Imtiaz F, Yasmeen A and Rathore HA (2022) Antihypertensive potential of Brassica rapa leaves: An in vitro and in silico approach. Front. Pharmacol. 13:996755. doi: 10.3389/fphar.2022.996755
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85139967557&origin=inward
URIhttp://hdl.handle.net/10576/35430
AbstractAim: Plants contain many essential constituents and their optimization can result in the discovery of new medicines. One such plant is Brassica rapa that is commonly used as a vegetable to fulfill daily food requirements worldwide. This study intends to screen the phytochemicals, antihypertensive potential, GC-MS, and in silico analysis of the leaves of Brassica rapa. Methods: Powdered leaves were subjected to proximate analysis followed by estimation of primary metabolites. Extracts were obtained by hot and cold extraction and investigated for secondary metabolites. All crude extracts were screened for their antihypertensive potential using an angiotensin-converting enzyme (ACE) inhibition assay. GC-MS analysis was carried out to standardize the extract, and an antihypertensive metabolite was confirmed using an in silico approach. Results: Physicochemical evaluation resulted in moisture content (9.10% ± 0.1), total ash value (18.10% ± 0.6), and extractive values (water 9.46% ± 0.5 and alcohol soluble 4.99% ± 0.1), while phytochemical investigation revealed primary metabolites (total proteins 11.90 mg/g ± 0.9; total fats 3.48 mg/g ± 0.5; and total carbohydrates 57.45 mg/g ± 1.2). Methanol extract showed the highest number of secondary metabolites including polyphenols 93.63 mg/g ± 0.6; flavonoids 259.13 mg/g ± 0.6; and polysaccharides 56.63 mg/g ± 1.4, while water extract (70 mg/g ± 2) was rich in glycosaponins. Methanol extract showed the highest antihypertensive potential by inhibiting ACE (79.39%) amongst all extracts, compared to the standard drug captopril, which inhibited 85.81%. Standardization of methanol extract via GC-MS analysis revealed potent phytoconstituents, and a molecular docking study confirmed that oleic acid is the main antihypertensive metabolite. Conclusion: We conclude that leaves of Brassica rapa can successfully lower hypertension by inhibiting ACE, however; in vivo investigations are required to confirm this antihypertensive activity.
SponsorOpen access funding is provided by the Qatar National Library
Languageen
PublisherFrontiers Media
SubjectACE inhibitor and molecular docking
antihypertensive action
Brassica rapa
food
in silico
TitleAntihypertensive potential of Brassica rapa leaves: An in vitro and in silico approach
TypeArticle
Pagination1-15
Volume Number13
ESSN1663-9812
dc.accessType Open Access


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