عرض بسيط للتسجيلة

المؤلفAbu-Madi, Marawan
المؤلفMahmoud, Aly
المؤلفBehnke, Jerzy M.
المؤلفClark, C. Graham
المؤلفBalkhy, Hanan
تاريخ الإتاحة2015-11-22T08:53:11Z
تاريخ النشر2015-09-15
اسم المنشورParasites & Vectors
الاقتباسAbu-Madi M., Mahmoud A., Behnke Jerzy M., Clark C. Graham, Balkhy H. The distribution of Blastocystis subtypes in isolates from Qatar, Parasites & Vectors , Volume 8, 2015.
معرّف المصادر الموحدhttp://dx.doi.org/10.1186/s13071-015-1071-3
معرّف المصادر الموحدhttp://hdl.handle.net/10576/3826
الملخصBackground: Blastocystis is a common single-celled intestinal parasite of humans and other animals comprising at least 17 genetically distinct small subunit ribosomal RNA lineages (subtypes (STs)), nine of which have been found in humans. The geographic distribution of Blastocystis subtypes is variable, but the subtypes present in Qatar are at present unknown. Methods: Stool samples were collected from randomly selected, apparently healthy subjects arriving in Qatar for the first time. Blastocystis subtypes were determined by sequencing of the small subunit rRNA gene (SSU rDNA) PCR products. Phylogenetic analyses were done using Maximum Composite Likelihood method. Results: 71.1 % of samples were positive for Blastocystis infection based on PCR-detection methodology compared to only 6.9 % by microscopy. Prevalence of Blastocystis did not differ between the sexes nor between age classes. However, there was a regional difference in prevalence with subjects arriving from Africa showing the highest (87.6 %), those from Western Asia intermediate (68.6 %) and from Eastern Asia the lowest prevalence (67.6 %). Genetic analysis detected only three STs. ST3 was the most common (69.3 %) and ST2 was the rarest (3.5 %), while ST1 had a prevalence of 27.2 %. ST2 showed a regional variation, being absent from the 64 Western Asian Blastocystis-positive subjects. Both ST1 and ST3 showed significant differences in prevalence between the sexes. Conclusions: This is the first report exploring the distribution of Blastocystis subtypes in our region. We recommend that stool screening via microscopy for the presence of Blastocystis should be abandoned since it is extremely insensitive. In future, the prevalence of Blastocystis infections should be based on PCR methodology and we predict that in the years ahead diagnostic PCR will become the tool of choice. More work is needed to identify the full range of Blastocystis subtypes that circulate in our region.
راعي المشروعQatar National ResearchFund, Qatar Foundation and HMC grant # 11110/11
اللغةen
الناشرBioMed Central Ltd.
الموضوعBlastocystis
Real time-PCR
Subtype
Small subunit ribosomal DNA
Prevalence
Genotyping
Phylogenetic
العنوانThe distribution of Blastocystis subtypes in isolates from Qatar
النوعArticle
رقم المجلد8
dc.accessType Open Access


الملفات في هذه التسجيلة

Thumbnail

هذه التسجيلة تظهر في المجموعات التالية

عرض بسيط للتسجيلة