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المؤلفSiddig, Alaa
المؤلفWan Abdul Rahman, Wan Faiziah
المؤلفMohd Nafi, Siti Norasikin
المؤلفSulong, Sarina
المؤلفYahya, Maya Mazuwin
المؤلفAl-Astani Tengku Din, Tengku Ahmad Damitri
المؤلفRazali, Rozaimi
المؤلفMusa, Kamarul Imran
تاريخ الإتاحة2023-05-25T07:35:40Z
تاريخ النشر2023-01-12
اسم المنشورBiomedicines
المعرّفhttp://dx.doi.org/10.3390/biomedicines11010200
الاقتباسSiddig, A., Wan Abdul Rahman, W. F., Mohd Nafi, S. N., Sulong, S., Yahya, M. M., Al-Astani Tengku Din, T. A. D., ... & Musa, K. I. (2023). Comparing the Biology of Young Versus Old Age Estrogen-Receptor-Positive Breast Cancer through Gene and Protein Expression Analyses. Biomedicines, 11(1), 200.
معرّف المصادر الموحدhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85146762430&origin=inward
معرّف المصادر الموحدhttp://hdl.handle.net/10576/43450
الملخصBackground: Breast cancer developed at a young age (≤45 years) is hypothesized to have unique biology; however, findings in this field are controversial. Methods: We compared the whole transcriptomic profile of young vs. old-age breast cancer using DNA microarray. RNA was extracted from 13 fresh estrogen receptor (ER)-positive primary breast cancer tissues of untreated patients (7 = young age ≤45 years and 6 = old age ≥55 years). In silico validation for the differentially expressed genes (DEGs) by young-age patients was conducted using The Cancer Genome Atlas (TCGA) database. Next, we analyzed the protein expression encoded by two of the significantly down-regulated genes by young-age patients, Glycine N-acyltransferase-like 1 (GLYATL-1) and Ran-binding protein 3 like (RANBP3L), using immunohistochemical analysis in an independent cohort of 56 and 74 ER-positive pre-therapeutic primary breast cancer tissues, respectively. Results: 12 genes were significantly differentially expressed by young-age breast cancers (fold change >2 or <2- with FDR p-value < 0.05). TCGA data confirmed the differential expression of six genes. Protein expression analysis of GLYATL-1 and RANBP3L did not show heterogeneous expression between young and old-age breast cancer tissues. Loss of expression of GLYATL-1 was significantly (p-value 0.005) associated with positive lymph node status. Higher expression of RANBP3L was significantly associated with breast cancers with lower histopathological grades (p-value 0.038). Conclusions: At the transcriptomic level, breast cancer developed in young and old age patients seems homogenous. The variation in the transcriptomic profiles can be attributed to the other clinicopathological characteristics rather than the age of the patient.
راعي المشروع- Ministry of Higher Education Malaysia (MOHE) - the fundamental research grant scheme (FRGS), grant # [FRGS/1/2019/SKK08/USM/02/15].
اللغةen
الناشرMultidisciplinary Digital Publishing Institute (MDPI)
الموضوعbreast cancer
estrogen-receptor-positive
gene expression
GLYATL-1
RANBP3L
transcriptomic profile
young age
العنوانComparing the Biology of Young versus Old Age Estrogen-Receptor-Positive Breast Cancer through Gene and Protein Expression Analyses
النوعArticle
رقم العدد1
رقم المجلد11
ESSN2227-9059
dc.accessType Open Access


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