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AuthorSiddig, Alaa
AuthorWan Abdul Rahman, Wan Faiziah
AuthorMohd Nafi, Siti Norasikin
AuthorSulong, Sarina
AuthorYahya, Maya Mazuwin
AuthorAl-Astani Tengku Din, Tengku Ahmad Damitri
AuthorRazali, Rozaimi
AuthorMusa, Kamarul Imran
Available date2023-05-25T07:35:40Z
Publication Date2023-01-12
Publication NameBiomedicines
Identifierhttp://dx.doi.org/10.3390/biomedicines11010200
CitationSiddig, A., Wan Abdul Rahman, W. F., Mohd Nafi, S. N., Sulong, S., Yahya, M. M., Al-Astani Tengku Din, T. A. D., ... & Musa, K. I. (2023). Comparing the Biology of Young Versus Old Age Estrogen-Receptor-Positive Breast Cancer through Gene and Protein Expression Analyses. Biomedicines, 11(1), 200.
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85146762430&origin=inward
URIhttp://hdl.handle.net/10576/43450
AbstractBackground: Breast cancer developed at a young age (≤45 years) is hypothesized to have unique biology; however, findings in this field are controversial. Methods: We compared the whole transcriptomic profile of young vs. old-age breast cancer using DNA microarray. RNA was extracted from 13 fresh estrogen receptor (ER)-positive primary breast cancer tissues of untreated patients (7 = young age ≤45 years and 6 = old age ≥55 years). In silico validation for the differentially expressed genes (DEGs) by young-age patients was conducted using The Cancer Genome Atlas (TCGA) database. Next, we analyzed the protein expression encoded by two of the significantly down-regulated genes by young-age patients, Glycine N-acyltransferase-like 1 (GLYATL-1) and Ran-binding protein 3 like (RANBP3L), using immunohistochemical analysis in an independent cohort of 56 and 74 ER-positive pre-therapeutic primary breast cancer tissues, respectively. Results: 12 genes were significantly differentially expressed by young-age breast cancers (fold change >2 or <2- with FDR p-value < 0.05). TCGA data confirmed the differential expression of six genes. Protein expression analysis of GLYATL-1 and RANBP3L did not show heterogeneous expression between young and old-age breast cancer tissues. Loss of expression of GLYATL-1 was significantly (p-value 0.005) associated with positive lymph node status. Higher expression of RANBP3L was significantly associated with breast cancers with lower histopathological grades (p-value 0.038). Conclusions: At the transcriptomic level, breast cancer developed in young and old age patients seems homogenous. The variation in the transcriptomic profiles can be attributed to the other clinicopathological characteristics rather than the age of the patient.
Sponsor- Ministry of Higher Education Malaysia (MOHE) - the fundamental research grant scheme (FRGS), grant # [FRGS/1/2019/SKK08/USM/02/15].
Languageen
PublisherMultidisciplinary Digital Publishing Institute (MDPI)
Subjectbreast cancer
estrogen-receptor-positive
gene expression
GLYATL-1
RANBP3L
transcriptomic profile
young age
TitleComparing the Biology of Young versus Old Age Estrogen-Receptor-Positive Breast Cancer through Gene and Protein Expression Analyses
TypeArticle
Issue Number1
Volume Number11
ESSN2227-9059
dc.accessType Open Access


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