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المؤلفSharifi, Shahriar
المؤلفSaei, Amir Ata
المؤلفGharibi, Hassan
المؤلفMahmoud, Nouf N.
المؤلفHarkins, Shannon
المؤلفDararatana, Naruphorn
المؤلفLisabeth, Erika M.
المؤلفSerpooshan, Vahid
المؤلفVégvári, Ákos
المؤلفMoore, Anna
المؤلفMahmoudi, Morteza
تاريخ الإتاحة2023-08-02T08:03:01Z
تاريخ النشر2022-06-20
اسم المنشورACS Applied Bio Materials
المعرّفhttp://dx.doi.org/10.1021/acsabm.2c00109
الاقتباسACS Appl Bio Mater. 2022 Jun 20;5(6):2643-2663. doi: 10.1021/acsabm.2c00109. Epub 2022 May 11.
معرّف المصادر الموحدhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85130802160&origin=inward
معرّف المصادر الموحدhttp://hdl.handle.net/10576/46463
الملخصAlbumin-based hydrogels offer unique benefits such as biodegradability and high binding affinity to various biomolecules, which make them suitable candidates for biomedical applications. Here, we report a non-immunogenic photocurable human serum-based (HSA) hydrogel synthesized by methacryloylation of human serum albumin by methacrylic anhydride (MAA). We used matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, liquid chromatography-tandem mass spectrometry, as well as size exclusion chromatography to evaluate the extent of modification, hydrolytic and enzymatic degradation of methacrylated albumin macromer and its cross-linked hydrogels. The impacts of methacryloylation and cross-linking on alteration of inflammatory response and toxicity were evaluated in vitro using brain-derived HMC3 macrophages and Ex-Ovo chick chorioallantoic membrane assay. Results revealed that the lysines in HSA were the primary targets reacting with MAA, though modification of cysteine, threonine, serine, and tyrosine, with MAA was also confirmed. Both methacrylated HSA and its derived hydrogels were nontoxic and did not induce inflammatory pathways, while significantly reducing macrophage adhesion to the hydrogels; one of the key steps in the process of foreign body reaction to biomaterials. Cytokine and growth factor analysis showed that albumin-based hydrogels demonstrated anti-inflammatory response modulating cellular events in HMC3 macrophages. Ex-Ovo results also confirmed the biocompatibility of HSA macromer and hydrogels along with slight angiogenesis-modulating effects. Photocurable albumin hydrogels may be used as a non-immunogenic platform for various biomedical applications including passivation coatings.
اللغةen
الناشرAmerican Chemical Society
الموضوعalbumin
hydrogels
inflammatory pathways
mass spectrometry
non-immunogenic
photocurable gels
structural analysis
العنوانMass Spectrometry, Structural Analysis, and Anti-Inflammatory Properties of Photo-Cross-Linked Human Albumin Hydrogels
النوعArticle
رقم العدد6
رقم المجلد5
dc.accessType Open Access


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