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المؤلفIppolito, Luigi
المؤلفMarini, Alberto
المؤلفCavallini, Lorenzo
المؤلفMorandi, Andrea
المؤلفPietrovito, Laura
المؤلفPintus, Gianfranco
المؤلفGiannoni, Elisa
المؤلفSchrader, Thomas
المؤلفPuhr, Martin
المؤلفChiarugi, Paola
المؤلفTaddei, Maria Letizia
تاريخ الإتاحة2016-10-16T09:21:40Z
تاريخ النشر2016-08-16
اسم المنشورOncotarget
المعرّفhttp://dx.doi.org/10.18632/oncotarget.11301
الاقتباسIppolito, L., Marini, A., Cavallini, L., Morandi, A., Pietrovito, L., Pintus, G., Giannoni, E., Schrader, T., Puhr, M., Chiarugi, P., & Letizia Taddei, M. (2016). Metabolic shift toward oxidative phosphorylation in docetaxel resistant prostate cancer cells. Oncotarget, 7(38), 61890-61904.
معرّف المصادر الموحدhttp://hdl.handle.net/10576/4888
الملخصDrug resistance of cancer cells is recognized as the primary cause of failure of chemotherapeutic treatment in most human cancers. Growing evidences support the idea that deregulated cellular metabolism is linked to such resistance. Indeed, both components of the glycolytic and mitochondrial pathways are involved in altered metabolism linked to chemoresistance of several cancers. Here we investigated the drug-induced metabolic adaptations able to confer advantages to docetaxel resistant prostate cancer (PCa) cells. We found that docetaxel-resistant PC3 cells (PC3-DR) acquire a pro-invasive behavior undergoing epithelial-to-mesenchymal-transition (EMT) and a decrease of both intracellular ROS and cell growth. Metabolic analyses revealed that PC3-DR cells have a more efficient respiratory phenotype than sensitive cells, involving utilization of glucose, glutamine and lactate by the mitochondrial oxidative phosphorylation (OXPHOS). Consequently, targeting mitochondrial complex I by metformin administration, impairs proliferation and invasiveness of PC3-DR cells without effects on parental cells. Furthermore, stromal fibroblasts, which cause a "reverse Warburg" phenotype in PCa cells, reduce docetaxel toxicity in both sensitive and resistant PCa cells. However, re-expression of miR-205, a microRNA strongly down-regulated in EMT and associated to docetaxel resistance, is able to shift OXPHOS to a Warburg metabolism, thereby resulting in an elevated docetaxel toxicity in PCa cells. Taken together, these findings suggest that resistance to docetaxel induces a shift from Warburg to OXPHOS, mandatory for conferring a survival advantage to resistant cells, suggesting that impairing such metabolic reprogramming could be a successful therapeutic approach.
راعي المشروعAssociazione Italiana Ricerca sul Cancro (AIRC), Istituto Toscano Tumori and Regione Toscana
اللغةen
الناشرImpact Journals
الموضوعprostate cancer
chemoresistance
docetaxel
oxidative phosphorylation
epithelial mesenchymal transition
العنوانMetabolic shift toward oxidative phosphorylation in docetaxel resistant prostate cancer cells
النوعArticle
الصفحات61890-61904
رقم العدد38
رقم المجلد7
ESSN1949-2553
dc.accessType Open Access


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