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AuthorKhan, Iftikhar
AuthorYousaf, Sakib
AuthorSubramanian, Sneha
AuthorKorale, Oshadie
AuthorAlhnan, Mohamed Albed
AuthorAhmed, Waqar
AuthorTaylor, Kevin M.G.
AuthorElhissi, Abdelbary
Available date2017-06-05T08:10:11Z
Publication Date2015-12-30
Publication NameInternational Journal of Pharmaceutics
Identifierhttp://dx.doi.org/10.1016/j.ijpharm.2015.10.002
CitationIftikhar Khan, Sakib Yousaf, Sneha Subramanian, Oshadie Korale, Mohamed Albed Alhnan, Waqar Ahmed, Kevin M.G. Taylor, Abdelbary Elhissi, Proliposome powders prepared using a slurry method for the generation of beclometasone dipropionate liposomes, International Journal of Pharmaceutics, Volume 496, Issue 2, 30 December 2015, Pages 342-350
ISSN03785173
URIhttp://www.sciencedirect.com/science/article/pii/S0378517315302696
URIhttp://hdl.handle.net/10576/5547
AbstractA novel “slurry method” was described for the preparation of proliposome powders using soya phosphatidylcholine (SPC) with cholesterol (1:1) and for incorporation of beclometasone dipropionate (BDP) at 2mole% of the total lipid phase. Proliposomes made with a range of lipid to sucrose carrier ratios were studied in terms of surface morphology using scanning electron microscopy (SEM) and thermal properties using differential scanning calorimetry (DSC). Following hydration of proliposomes, the resultant vesicles were compared to liposomes made using the traditional proliposome method, in terms of vesicle size and drug entrapment efficiency. SEM showed that sucrose was uniformly coated with lipid regardless of lipid to carrier ratio. Liposomes generated using the slurry proliposome method tended to have smaller median size than those generated with the conventional proliposome method, being in the range of 4.72–5.20μm and 5.89–7.72μm respectively. Following centrifugation of liposomes using deuterium oxide (D2O) as dispersion medium, vesicles entrapping BDP were separated as a floating creamy layer, whilst the free drug was sedimented as crystals. Drug entrapment was dependent on formulation composition and preparation method. When 1:15 w/w lipid to carrier was used, liposomes generated using the slurry method had an entrapment efficiency of 47.05% compared to 18.67% for those generated using the conventional proliposome method. By contrast, liposomes made by the thin-film hydration method had an entrapment efficiency of 25.66%. DSC studies using 50mole% BDP demonstrated that the drug was amorphous in the proliposome formulation and tended to crystallize on hydration, resulting in low drug entrapment. In conclusion, a novel approach to the preparation of proliposomes using a slurry method has been introduced, offering higher entrapment for BDP than liposomes made using the conventional proliposome method and those prepared by thin-film hydration technique.
Languageen
PublisherElsevier
SubjectCharacterization
Drug development
Proliposome
Liposome
Manufacture
TitleProliposome powders prepared using a slurry method for the generation of beclometasone dipropionate liposomes
TypeArticle
Pagination342-350
Issue Number2
Volume Number496
dc.accessType Abstract Only


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