Formulation optimization of lyophilized aptamer-gold nanoparticles: Maintained colloidal stability and cellular uptake
Author | Saidi, Dalya |
Author | Obeidat, Marya |
Author | Alsotari, Shrouq |
Author | Ibrahim, Abed-Alqader |
Author | Al-Buqain, Rula |
Author | Wehaibi, Suha |
Author | Alqudah, Dana A. |
Author | Nsairat, Hamdi |
Author | Alshaer, Walhan |
Author | Alkilany, Alaaldin M. |
Available date | 2024-09-23T06:45:21Z |
Publication Date | 2024 |
Publication Name | Heliyon |
Resource | Scopus |
ISSN | 24058440 |
Abstract | Anti-nucleolin (NCL) aptamer AS1411 is the first anticancer aptamer tested in clinical trials. Gold nanoparticles (AuNP) have been widely exploited for various biomedical applications due to their unique functional properties. In this study, we evaluated the colloidal stability and targeting capacity of AS1411-funtionalized AuNP (AuNP/NCL-Apt) against MCF-7 breast cancer cell line before and after lyophilization. Trehalose, mannitol, and sucrose at various concentrations were evaluated to determine their cryoprotection effects. Our results indicate that sucrose at 10 % (w/v) exhibits the best cryoprotection effect and minimal AuNP/NCL-Apt aggregation as confirmed by UV-Vis spectroscopy and dynamic light scattering (DLS) measurements. Moreover, the lyophilized AuNP/NCL-Apt at optimized formulation maintained its targeting and cytotoxic functionality against MCF-7 cells as proven by the cellular uptake assays utilizing flow cytometry and confocal laser scanning microscopy (CLSM). Quantitative PCR (qPCR) analysis of nucleolin-target gene expression also confirmed the effectiveness of AuNP/NCL-Apt. This study highlights the importance of selecting the proper type and concentration of cryoprotectant in the typical nanoparticle lyophilization process and contributes to our understanding of the physical and biological properties of functionalized nanoparticles upon lyophilization. |
Sponsor | This study was funded by the thesis-oriented fund granted by the deanship of research at Jordan University of science and technology (grant # 2020/0493). |
Language | en |
Publisher | Elsevier |
Subject | Aggregation Aptamer Cryoprotectants Gold nanoparticles Lyophilization |
Type | Article |
Issue Number | 10 |
Volume Number | 10 |
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Pharmacy Research [1318 items ]