تصفح حسب المؤلف "Tounsi, Slim"
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Agrotis segetum midgut putative receptor of Bacillus thuringiensis vegetative insecticidal protein Vip3Aa16 differs from that of Cry1Ac toxin
Ben Hamadou-Charfi, Dorra; Boukedi, Hanen; Abdelkefi-Mesrati, Lobna; Tounsi, Slim; Jaoua, Samir ( Elsevier Inc. , 2013 , Article)Considering the fact that Agrotis segetum is one of the most pathogenic insects to vegetables and cereals in the world, particularly in Africa, the mode of action of Vip3Aa16 of Bacillus thuringiensis BUPM95 and Cry1Ac of ... -
Amino acids Y229 and F603 are involved in Bacillus thuringiensis Cry1Ac delta-endotoxin stability and toxicity
Dammak, Mariam; Ali, Mamdouh Ben; Jaoua, Samir; Tounsi, Slim ( Blackwell Publishing , 2012 , Article)Bacillus thuringiensis Cry1Ac toxin shares structurally five conserved blocs with the other δ-endotoxins. To study the role of some amino acids belonging to these regions, two mutations, Y(229) P and F(603) S, were introduced ... -
Bayesian network and response surface methodology for prediction and improvement of bacterial metabolite production
Bouchaala, Lobna; Ben Khedher, Saoussen; Mezghanni, Héla; Zouari, Nabil; Tounsi, Slim ( Institute of Electrical and Electronics Engineers Inc. , 2015 , Conference Paper)The optimization of antifungal activity production by Bacillus amyloliquefaciens was carried out using Response Surface Methodology (RSM) in two steps. The first step involved the screening of cultural parameters affecting ... -
Characterisation of novel Bacillus thuringiensis isolates against Aedes aegypti (Diptera: Culicidae) and Ceratitis capitata (Diptera:Tephridae)
Elleuch, Jihen; Tounsi, Slim; Ben Hassen, Najeh Belguith; Lacoix, Marie Noel; Chandre, Fabrice; Jaoua, Samir; Zribi Zghal, Raida... more authors ... less authors ( Elsevier Inc. , 2015 , Article)Bacillus thuringiensis is successfully used in pest management strategies as an eco-friendly bioinsecticide. Isolation and identification of new strains with a wide variety of target pests is an ever growing field. In this ... -
Chemical composition and evaluation of antioxidant and antimicrobial activities of Tunisian Thymelaea hirsuta with special reference to its mode of action
Trigui, Mohamed; Ben Hsouna, Anis; Tounsi, Slim; Jaoua, Samir ( Elsevier B.V. , 2013 , Article)In the present study, we evaluated in vitro antimicrobial and antioxidant properties of Thymelaea hirsuta extracts as well as its phytochemical composition. Total phenolic and flavonoid contents were highest in acetone and ... -
Combinatorial effect of mutagenesis and medium component optimization on Bacillus amyloliquefaciens antifungal activity and efficacy in eradicating Botrytis cinerea
Masmoudi, Fatma; Khedher, Saoussen Ben; Kamoun, Amel; Zouari, Nabil; Tounsi, Slim; Trigui, Mohamed... more authors ... less authors ( Elsevier GmbH , 2017 , Article)This work is directed towards Bacillus amyloliquefaciens strain BLB371 metabolite production for biocontrol of fungal phytopathogens. In order to maximise antifungal metabolite production by this strain, two approaches ... -
Comparative study of Bacillus thuringiensis Cry1Ia and Cry1Aa delta-endotoxins : activation process and toxicity against Prays oleae
Dammak, Mariam; Tounsi, Slim; Rousis, Souad; Jaoua, Samir ( Elsevier Inc. , 2010 , Article)Cry1Ia and Cry1Aa proteins exhibited toxicities against Prays oleae with LC(50) of 189 and 116 ng/cm(2), respectively. The ability to process Cry1Ia11 protoxin by trypsin, chymotrypsin and P. oleae larvae proteases was ... -
Construction of a Bacillus thuringiensis genetically-engineered strain harbouring the secreted Cry1Ia delta-endotoxin in its crystal
Dammak, Mariam; Jaoua, Samir; Tounsi, Slim ( Springer Netherlands , 2011 , Article)Unlike other Bacillus thuringiensis Cry proteins, Cry1 Ia does not form a crystal since it is a secreted delta-endotoxin. We have engineered a Cry1 Iac chimeric protein by substituting the C-terminal part of Cry1 Ia by the ... -
Cry1Ac toxicity enhancement towards lepidopteran pest Ephestia kuehniella through its protection against excessive proteolysis
Elleuch, Jihen; Jaoua, Samir; Tounsi, Slim; Zghal, Raida Z. ( Elsevier , 2016 , Article)Bacillus thuringiensis has been extensively used in agroecosystems for four decades due to its high specific toxicity. Strategies based on B. thuringiensis proteins combinations for the improvement of its activity present ... -
Cry4Ba and Cyt1Aa proteins from Bacillus thuringiensis israelensis: Interactions and Toxicity mechanism against Aedes aegypti
Elleuch, Jihen; Jaoua, Samir; Darriet, Frederic; Chandre, Fabrice; Tounsi, Slim; Zribi Zghal, Raida... more authors ... less authors ( Elsevier Ltd. , 2015 , Article)Individual crystal proteins from Bacillus thuringiensis israelensis exhibit variable levels of insecticidal activities against mosquito larvae. In all cases, they are much less active compared to the whole crystal proteins ... -
Efficacy of Lawsonia inermis leaves extract and its phenolic compounds against olive knot and crown gall diseases.
Mohamed, T.; Ben Hsouna, Anis; Hammami, Inès; Culioli, Gérald; Ksantini, Mohieddine; Tounsi, Slim; Jaoua, Samir... more authors ... less authors ( Elsevier , 2013 , Article)This study was undertaken to determine the antibacterial efficacy of the leaves extracts of Lawsonia inermis in vitro and in vivo, against the phytopathogenic bacteria Pseudomonas savastanoi pv savastanoi IVIA 1628 and ... -
Erratum to: Towards novel Cry toxins with enhanced toxicity/broader: a new chimeric Cry4Ba / Cry1Ac toxin
Zribi Zghal, Raida; Elleuch, Jihen; Ben Ali, Mamdouh; Darriet, Frédéric; Rebaï, Ahmed; Chandre, Fabrice; Jaoua, Samir; Tounsi, Slim... more authors ... less authors ( Springer Verlag , 2017 , Other)The original version of this article inadvertently contained mistake. 1 Springer-Verlag GmbH Germany 2017 -
Evidence of two mechanisms involved in Bacillus thuringiensis israelensis decreased toxicity against mosquito larvae: Genome dynamic and toxins stability
Elleuch, Jihen; Zribi Zghal, Raida; Lacoix, Marie Noel; Chandre, Fabrice; Tounsi, Slim; Jaoua, Samir... more authors ... less authors ( Elsevier GmbH , 2015 , Article)Biopesticides based on Bacillus thuringiensis israelensis are the most used and most successful around the world. This bacterium is characterized by a dynamic genome able to win or lose genetic materials which leads to a ... -
Halobacteria-Based Biofertilizers: A Promising Alternative for Enhancing Soil Fertility and Crop Productivity under Biotic and Abiotic Stresses—A Review
Masmoudi, Fatma; Alsafran, Mohammed; Jabri, Hareb A.L.; Hosseini, Hoda; Trigui, Mohammed; Sayadi, Sami; Tounsi, Slim; Saadaoui, Imen... more authors ... less authors ( MDPI , 2023 , Other)Abiotic and biotic stresses such as salt stress and fungal infections significantly affect plant growth and productivity, leading to reduced crop yield. Traditional methods of managing stress factors, such as developing ... -
Histopathological effects and determination of the putative receptor of Bacillus thuringiensis Cry1Da toxin in Spodoptera littoralis midgut
Dalel, BenFarhat-Touzri; Saadaoui, Marwa; Abdelkefi-Mesrati, Lobna; Saadaoui, Imen; Azzouz, Hichem; Tounsi, Slim... more authors ... less authors ( Elsevier , 2012 , Article)Bacillus thuringiensis subsp. aizawai strain HD133, known by its effectiveness against Spodoptera species, produces many insecticidal proteins including Cry1Ab, Cry1Ca and Cry1Da. In the present study, the insecticidal ... -
Integration of a Recombinant Chitinase into Bacillus thuringiensis Parasporal Insecticidal Crystal
Driss, Fatma; Rouis, Souad; Azzouz, Hichem; Tounsi, Slim; Zouari, Nabil; Jaoua, Samir... more authors ... less authors ( Springer-Verlag , 2011 , Article)Chitinases have been successfully used in combination with Bacillus thuringiensis delta-endotoxins forming crystals in order to enhance their insecticidal activities. In this context, we opted for promoting the chitinase ... -
Isolation of the Bacillus thuringiensis plasmid carrying Bacthuricin F4 coding genes and evidence of its conjugative transfer
Ben Fguira, Ines; Fourati, Zaineb; Kamoun, Fakher; Tounsi, Slim; Jaoua, Samir ( The Journal of Infection in Developing Countries , 2014 , Article)INTRODUCTION: Conjugation is an excellent natural mode of DNA transfer in vivo between bacteria, particularly when these conjugative elements carry technological traits such as bacteriocin encoding genes. In the present ... -
New Bacillus thuringiensis toxin combinations for biological control of Lepidopteran larvae
Elleuch, Jihen; Zribi Zghal, Raida; Jemaa, Mohamed; Azzouz, Hichem; Tounsi, Slim; Jaoua, Samir... more authors ... less authors ( Elsevier B.V. , 2014 , Article)Cyt1Aa from Bacillus thuringiensis israelensis is known by its synergistical activity with B. thuringiensis and Bacillus sphaericus toxins. It is able to improve dipteran specific toxins activity and can prevent or overcome ... -
Overproduction of the Bacillus thuringiensis Vip3Aa16 toxin and study of its insecticidal activity against the carob moth Ectomyelois ceratoniae
Hanen, Boukedi; Ben Khedher, Saoussen; Triki, Nesrine; Kamoun, Fakher; Saadaoui, Imen; Chakroun, Maissa; Tounsi, Slim; Abdelkefi-Mesrati, Lobna... more authors ... less authors ( Elsevier , 2015 , Article)The vip3Aa16 gene of Bacillus thuringiensis strain BUPM95 was cloned and expressed in Escherichia coli. Optimization of Vip3A16 protein expression was conducted using Plackett–Burman design and response surface methodology. ... -
Potential of Photorhabdus temperata K122 bioinsecticide in protecting wheat flour against Ephestia kuehniella
Jallouli, Wafa; Abdelkefi-Mesrati, Lobna; Tounsi, Slim; Jaoua, Samir; Zouari, Nabil ( Elsevier Ltd. , 2013 , Article)The present study reports investigations on the insecticidal activity of the entomopathogenic bacterium Photorhabdus temperata K122 against the Mediterranean flour moth Ephestia kuehniella. Cultured in the optimized medium, ...