Characterization Of Genomic Alterations And Tcrβ Repertoire Of Tumor- Infiltrating Lymphocytes In Breast Cancer

Abstract

Breast cancer still remains a major cause of morbidity and mortality among women in Qatar and worldwide. More recent studies indicate that the diversity and the composition of the entire set of antigen receptors within tumor-infiltrating lymphocytes (TILs) is strongly correlated with tumor prognosis and therapeutic response with breast cancer. Unfortunately, the relationship between somatic mutational load and TCR diversity of TILs across breast cancer still limited. For this purpose, first we characterized the somatic mutations of Formalin-Fixed Paraffin-Embedded breast cancer samples from 79 patients using NGS of a panel of cancer related genes. Second, we classified and identified the TCRß repertoire for these 11 samples using the ImmunoSEQ platform. Preliminary data demonstrated that the 11 patients had high diversity of TCRß-CDR3 within the tumors. However, there was no statistically significant association between the somatic mutational loads in the gene panels we sequenced and the number of productive TCRß-CDR3 rearrangements.