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AuthorSadek, Khaled W
AuthorHaik, Mahmoud Y
AuthorAshour, Anas A
AuthorBaloch, Tahira
AuthorAboulkassim, Tahar
AuthorYasmeen, Amber
AuthorVranic, Semir
AuthorZeidan, Asad
AuthorAl Moustafa, Ala-Eddin
Available date2019-04-02T11:22:36Z
Publication Date2018-11-13
Publication NameCancer Cell International
Identifierhttp://dx.doi.org/10.1186/s12935-018-0678-9
CitationSadek et al. Water-pipe smoking promotes epithelial– mesenchymal transition and invasion of human breast cancer cells via ERK1/ERK2 pathways. Cancer Cell International. 2018 . Volume 18
URIhttp://hdl.handle.net/10576/11466
AbstractWith the increasing popularity of water-pipe smoking (WPS), it is critical to comprehend how WPS may affect women's health. The main goal of this study is to identify the potential outcome of WPS on human breast cancer progression. Two breast cancer cell lines, MCF7 and BT20, were used in this investigation. We explored the outcome of WPS on cell morphology and cell invasion using inverted microscope and Biocoat Matrigel invasion chambers. On the other hand, Western blot was employed to study the expression patterns of key control genes of cell adhesion and invasion. Our data reveal that WPS induces epithelial-mesenchymal transition (EMT) of MCF7 and BT20 breast cancer cell lines; thus, WPS enhances cell invasion ability of both cell lines in comparison with their matched controls. More significantly, WPS provokes a down- and up-regulation of E-cadherin and focal adhesion kinase (FAK), respectively, which are important key regulators of cancer progression genes. Finally, our data point out that WPS incites the activation of Erk1/Erk2, which could be behind the stimulation of EMT and invasion as well as the deregulation of E-cadherin and FAK expression. Our data show, for the first time, that WPS initiates EMT and stimulates cell invasion of breast cancer cells, which could incite metastatic development in breast cancer patients. Thus, we believe that further studies, both in vitro and in vivo, are required to elucidate the pathogenic outcome of WPS on cancer progression of several human carcinomas including breast.
Languageen
PublisherBioMed Central Ltd.
SubjectBreast cancer
Cell adhesion
Cell invasion
EMT
Erk1/Erk2 pathways
Water-pipe smoking
TitleWater-pipe smoking promotes epithelial-mesenchymal transition and invasion of human breast cancer cells via ERK1/ERK2 pathways.
TypeArticle
Volume Number18
ESSN1475-2867
dc.accessType Open Access


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