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AuthorZhang, Mengtian
AuthorWang, Qin
AuthorWan, Ka-Wai
AuthorAhmed, Waqar
AuthorPhoenix, David A
AuthorZhang, Zhirong
AuthorElrayess, Mohamed A
AuthorElhissi, Abdelbary
AuthorSun, Xun
Available date2019-08-22T11:56:22Z
Publication Date2019-04-29
Publication NameInternational Journal of Pharmaceutics
Identifierhttp://dx.doi.org/10.1016/j.ijpharm.2019.04.078
CitationM. Zhang, Q. Wang, K-W. Wan, W. Ahmed, D.A. Phoenix, Z. Zhang, M.A. Elrayess, A. Elhissi, X. Sun, Liposome Mediated-CYP1A1 Gene Silencing Nanomedicine Prepared Using Lipid Film-Coated Proliposomes as a Potential Treatment Strategy of Lung Cancer, International Journal of Pharmaceutics (2019), doi: https://doi.org/10.1016/j.ijpharm.2019.04.078
ISSN0378-5173
IdentifierPII: S0378-5173(19)30349-7
IdentifierReference: IJP 18326
URIhttp://hdl.handle.net/10576/11707
AbstractThe occurrence of lung cancer is linked with tobacco smoking, mainly through the generation of polycyclic aromatic hydrocarbons (PAHs). Elevated activity of cytochrome P4501A1 (CYP1A1) plays an important role in the metabolic processing of PAHs and its carcinogenicity. The present work aimed to investigate the role of CYP1A1 gene in PAH-mediated growth and tumor development in vitro and using an in vivo animal model. RNAi strategy was utilized to inhibit the overexpression of CYP1A1 gene using cationic liposomes generated using a lipid film-coated proliposome microparticles. Treatment of PAH-induced human alveolar adenocarcinoma cell line with cationic liposomes carrying CYP1A1 siRNA resulted in down regulation of CYP1A1 mRNA, protein as well as its enzymatic activity, triggering apoptosis and inhibiting multicellular tumor spheroids formation in vitro. Furthermore, silencing of CYP1A1 gene in BALB/c nude xenografts inhibited tumor growth via down regulation of CYP1A1 expression. Altogether, our findings showed that liposome-based gene delivery technology is a viable and stable approach for targeting cancer causing genes such as CY1PA1. This technology facilitated by the use of sugar particles coated with lipid films has demonstrated ability to generate anticancer effects that might be used in the future for therapeutic intervention and treatment of lung cancer.
SponsorBiomedical Technology (Shenzhen) Ltd, China and Qatar University
Languageen
PublisherElsevier
SubjectApoptosis
SubjectCYP1A1
SubjectCancer
SubjectLung
SubjectSmoking
SubjectTobacco
SubjectsiRNA
TitleLiposome mediated-CYP1A1 gene silencing nanomedicine prepared using lipid film-coated proliposomes as a potential treatment strategy of lung cancer.
TypeArticle
Pagination185-193
Volume Number566
ESSN1873-3476


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