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AuthorNagi, Karim
AuthorShenoy, Sudha K
Available date2019-09-15T05:56:12Z
Publication Date2019
Publication NameBeta-Arrestinsen_US
Identifier10.1007/978-1-4939-9158-7_6
CitationNagi K., Shenoy S.K. (2019) Detection of β-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination Using BRET. In: Scott M., Laporte S. (eds) Beta-Arrestins. Methods in Molecular Biology, vol 1957. Humana Press, New York, NY
ISBN978-1-4939-9157-0
ISBN978-1-4939-9158-7
URIhttp://hdl.handle.net/10576/11831
AbstractUbiquitination of G protein-coupled receptors (GPCRs) is an important dynamic posttranslational modification that has been linked to the intracellular trafficking of internalized GPCRs to lysosomes. Ubiquitination of GPCRs is mediated by specific E3 ubiquitin ligases that are scaffolded by the adaptor proteins called β-arrestins. Traditionally, detection of GPCR ubiquitination is achieved by using ubiquitin antibodies to Western blot immunoprecipitates of detergent-solubilized GPCRs expressed in heterologous cells. However, studies have also shown that bioluminescence resonance energy transfer (BRET)-based techniques can reveal ubiquitination of GPCRs in intact cells and in real time. This chapter describes a step-by-step protocol to evaluate ubiquitination of GPCRs using the BRET methodology.
Languageen
PublisherHumana Press, New York, NY
SubjectAdrenergic receptor
SubjectArrestin
SubjectBRET
SubjectGPCR
SubjectTitration
SubjectUbiquitin
SubjectUbiquitination
TitleDetection of β-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination Using BRET.
TypeBook chapter
Pagination93-104


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