Show simple item record

AuthorHammad Y.
AuthorElmoghazy W.
AuthorEl Ansari W.
AuthorLance M.
AuthorZaghw A.
AuthorShallik N.
Available date2020-04-01T06:59:41Z
Publication Date2019
Publication NameIndian Journal of Anaesthesia
ResourceScopus
ISSN195049
URIhttp://dx.doi.org/10.4103/ija.IJA_398_19
URIhttp://hdl.handle.net/10576/13665
AbstractBackground and Aims: Human plasma protein fraction 5% (PPF5%) is an albumin-based colloid used to expand the plasma volume during volume deficiency. The current basic medical experimental study assessed in vitro coagulation of PPF5% solution and its effects on blood coagulation and chemistry. Methods: The study involved 20 volunteers, and each volunteer donated 20-50 ml of fresh blood. Three dilutions of blood with PPF5% dilutions were prepared (30, 50, and 70%). The fibrinogen dose required to correct coagulation in the 50% diluted samples was assessed (two doses used). The thromboelastogram (TEG) measured the haemostatic parameters (fibrinogen level, initiation of coagulation [R time], kinetics [K], acceleration of coagulation [? angle], maximum amplitude [MA] and coagulation index [CI]), and the ABL gas analyser measured the blood chemistry changes. Results: All dilutions showed significant TEG and blood chemistry changes when compared to controls. The two doses of fibrinogen corrected the clot formation speed with no significant difference in speed between the two doses. Acidosis measured by the strong ion gap (SID) and pH were significant for all dilutions when compared with the baseline. The 30% dilution remained within the lower normal acceptable value while 50% dilution was beyond the critical normal values. Conclusion: In vitro PPF5% to replace blood loss up to 50% dilution did not have significant coagulation and blood chemistry effects while coagulopathy should be expected in extreme dilutions (70%). Fibrinogen in a dose equivalent to 4 gm/70 kg adult improved clot strength at 50% dilution. - 2019 Wolters Kluwer Medknow Publications. All rights reserved.
Languageen
PublisherWolters Kluwer Medknow Publications
SubjectBlood coagulation and chemistry
fibrinogen level
human plasma protein fraction
thromboelastography
TitleExperimental effect of different dilutions of blood with human plasma protein fraction and large dose factor one on blood coagulation and chemistry in vitro
TypeArticle
Pagination1015-1021
Issue Number12
Volume Number63
dc.accessType Open Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record