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AuthorFatiha M., Benslimane
AuthorZakaria, Zain Z.
AuthorShurbaji, Samar
AuthorAbdelrasool, Mahmoud Khatib Ali
AuthorAl-Badr, Mashael Ali H.I.
AuthorAl Absi, Enas Said Khalil
AuthorYalcin, Huseyin C.
Available date2020-06-09T07:35:05Z
Publication Date2020-09-30
Publication NameMicron
Identifierhttp://dx.doi.org/10.1016/j.micron.2020.102876
CitationBenslimane, Fatiha & Zakaria, Zain & Shur, Samar & Al-Badr, Mashael & Absi, Enas & Yalcin, Huseyin & Al-Ruweidi, Mahmoud. (2020). Cardiac function and blood flow hemodynamics assessment of zebrafish (Danio rerio) using high-speed video microscopy. Micron. 102876. 10.1016/j.micron.2020.102876.
ISSN09684328
URIhttp://hdl.handle.net/10576/15023
AbstractBackgroundIn the last few decades, zebrafish (Danio rerio) were introduced as a model organism to investigate human diseases including cardiovascular and neuronal disorders. In most zebrafish investigations, cardiac function and blood flow hemodynamics need to be assessed to study the effects of the interference on the cardiovascular system. For heart function assessment, most important parameters include heart rate, cardiac output, ejection fraction, fractional area change, and fractional shortening. MethodsA 10 s high-speed video of beating heart and flowing blood within major vessels of zebrafish that are less than 5 days post fertilization (dpf) were recorded via a stereo microscope equipped with a high speed camera. The videos were analyzed using MicroZebraLab and image J software for the assessment of cardiac function. ResultsUsing the technique described here, we were able to simply yet effectively assess cardiac function and blood flow dynamics of normal zebrafish embryos. We believe that the practical method presented here will help cardiac researchers using the zebrafish as a model to examine cardiac function by using tools that could be available in their laboratory.
SponsorThis work is supported by Qatar National Research Fund (QNRF),National Priority Research Program under grant number NPRP 10-0123-170222.
Languageen
PublisherElsevier
SubjectZebrafish embryo
Cardiac function
Hemodynamics
Blood flow
Shear stress
MicroZebraLab
ImageJ
TitleCardiac function and blood flow hemodynamics assessment of zebrafish (Danio rerio) using high-speed video microscopy
TypeArticle
Volume Number136


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