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AuthorEnnouri, Karim
AuthorBen Hassen, Hanen
AuthorZouari, Nabil
Available date2020-09-20T08:35:36Z
Publication Date2017
Publication NameProceedings of the National Academy of Sciences India Section B - Biological Sciences
ResourceScopus
ISSN3698211
URIhttp://dx.doi.org/10.1007/s40011-015-0620-z
URIhttp://hdl.handle.net/10576/16157
AbstractBacillus thuringiensiskurstaki is a soil bacterium that produces insecticidal toxins called delta-endotoxins. In order to increase the toxic crystal concentrations in a low-cost culture medium and thus improve the biopesticide quality to control insect pests, the Plackett Burman screening method was applied. It was shown a tool to evaluate the significance of the selected seven factors (KH2PO4, K2HPO4, MgSO4, MnSO4, FeSO4, soybean meal, starch) which are necessary to the production of the delta-endotoxins. This was performed into two different shake flasks (250 and 500 ml). The main factors that affected the production of delta-endotoxins are shown to be soybean meal, starch, and FeSO4 in 250 ml culture flasks. In 500 ml culture flasks, soybean meal and FeSO4 are the principal factors influencing the delta-endotoxin production. The multiple linear regression, a method applied as the merging dataset of the two Plackett Burman designs, established that soybean meal and starch are the factors positively affecting the production of delta-endotoxins, in contrast to FeSO4. Furthermore, the available oxygen in culture flasks showed no significant negative effect on delta-endotoxin production. This study revealed that mixed method designs were useful to identify the significance and the effect of hidden culture parameters.
Languageen
PublisherSpringer India
SubjectBacillus thuringiensis kurstaki
Culture medium
Insecticidal toxins
Mixed Plackett Burman designs
Multiple linear regression
TitleNutritional Requirements to Improve Delta-Endotoxins Production of Bacillus thuringiensis var. kurstaki Using Mixed Designs Modelling
TypeArticle
Pagination307-314
Issue Number2
Volume Number87
dc.accessType Abstract Only


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