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AuthorSultan, Ahmed
AuthorYang, Keun-Hang Susan
AuthorIsaev, Dmitro
AuthorElNebrisi, Eslam
AuthorSyed, Nurulain
AuthorKhan, Nadia
AuthorHowarth, Christopher F.
AuthorSadek, Bassem
AuthorOzad, Murat
Available date2020-09-20T08:35:37Z
Publication Date2017
Publication NameToxicology
ISSN0300483X
URIhttp://dx.doi.org/10.1016/j.tox.2017.04.005
URIhttp://hdl.handle.net/10576/16172
AbstractEffects of thujone, a major ingredient of absinthe, wormwood oil and some herbal medicines, were tested on the function of 7 subunit of the human nicotinic acetylcholine (7 nACh) receptor expressed in Xenopus oocytes using the two-electrode voltage-clamp technique. Thujone reversibly inhibited ACh (100 M)-induced currents with an IC50 value of 24.7 M. The effect of thujone was not dependent on the membrane potential and did not involve Ca2+-dependent Cl channels expressed endogenously in oocytes. Inhibition by thujone was not reversed by increasing ACh concentrations. Moreover, specific binding of [125I] -bungarotoxin was not altered by thujone. Further experiments in SH-EP1 cells expressing human 7 nACh receptor indicated that thujone suppressed choline induced Ca2+ transients in a concentration-dependent manner. In rat hippocampal CA3-dentate gyrus synapses, nicotine-induced enhancement of long-term potentiation was also inhibited by thujone. Furthermore, the results observed in in-vivo one-trial passive avoidance paradigm show that thujone (1.25 mg/kg, i.p.) significantly impaired nicotine-induced enhancement of learning and memory in Wistar rats. Collectively, our results indicate that thujone inhibits the function of the 7-nACh receptor and impairs cellular and behavioral correlates of cholinergic modulation of learning and memory.
Languageen
PublisherElsevier Ireland Ltd
SubjectAbsinthe
Subjectmemory
SubjectNicotinic receptors
Subjectpassive avoidance paradigm
SubjectThujone
SubjectXenopus oocyte
TitleThujone inhibits the function of 7-nicotinic acetylcholine receptors and impairs nicotine-induced memory enhancement in one-trial passive avoidance paradigm
TypeArticle
Pagination23-32
Volume Number384


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